Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1985-02-15
1988-04-26
Hazel, Blondel
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436511, 436548, G01N 5300, G01N 33511, G01N 3353
Patent
active
047404671
ABSTRACT:
Murine anti-Treponema pallidum monoclonal antibodies were employed in the detection of low numbers of pathogenic treponemes. Monoclonal antibodies were used as a primary antibody source in a solid-phase immunoblot assay system. All monoclonal antibodies assayed were capable of detecting ca. 1.0.times.10.sup.3 to 2.5.times.10.sup.3 treponemes. Of 13 monoclonal antibodies examined, 3 were able to detect 10.sup.3 virulent treponemes, and 1 of these antibodies was able to reveal the presence of as few as 500 organisms. Western blot analyses showed that all anti-T. pallidum monoclonal antibodies exhibiting high sensitivities for the detection of T. pallidum cells were directed against an abundant, 47,000-48,000 dalton surface-exposed antigen of the organism. With two possible exceptions, the monoclonal antibodies tested reacted specifically with T. pallidum, either purified or found within a high-contaminating tissue background, and not with Treponema phagedenis biotype Reiter, Haemophilus ducreyi, Neisseria gonorrhoeae, herpes simplex virus type 2, or normal rabbit testicular tissue.
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The Government may have rights in this invention pursuant to National Institutes of Health Grant Nos. AI16682, AI11851 and CA-23115 awarded by the Department of Health and Human Services.
Kettman John R.
Norgard Michael V.
Board of Regents The University of Texas
Hazel Blondel
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