Methods for determining the presence or absence of elite...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C536S024300

Reexamination Certificate

active

06900014

ABSTRACT:
This invention pertains to methods for determining the presence or absence inBrassicaplant material of elite event MS-B2, which is a specific transformation event of a construct comprising a male-sterility gene, at a specific location in theBrassicagenome, using PCR and primers specific to the construct, and kits for use in the methods.

REFERENCES:
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patent: WO 91 02069 (1991-02-01), None
patent: WO 95 34634 (1995-12-01), None
patent: WO 96 26283 (1996-08-01), None
Knapp et al. 1994. Transgenic tomato lines containing Ds elements at defined genomic positions as tools for targeted transposon tagging. Mol. Gen. Genet. 243:666-673.*
Eshed et al. 1996. Less-than-additive epistatic interactions of quantitative tait loci in tomato. Gentics 143:1807-1817.*
Mariani et al. 1990. Induction of male sterility in plants by chimaeric ribonuclease gene. Nature 347:737-741.*
Reynaerts et al. 1993. Engineered genes for fertility control and their application in hybrid seed production. Scientia Horticulturae 55:125-139.*
Rong et al. 1996. Induction of male sterility inBrassica napusby TA29-barnase gene. Acta Botanica Sinica 38(7):582-585.*
Goring et al. 1991. Transformation of a partial nopaline synthase gene into tobacco suppresses the expression of a resident wild-type gene. Proc. Natl. Acad. Sci. (USA) 88:1770-1774.*
Thomas et al. 1994. Analysis of the chromosomal distribution of transposon-carrying T-DNAs in tomato using the inverse polymerase chain reaction. Mol. Gen. Genet. 242:573-585.*
Hartley R. W., “Barnase and Barstar Expression of Its Cloned Inhibitor Permits Expression of a Cloned Ribonuclease”,Journal of Molecular Biology, vol. 202, No. 4, 1988, pp. 913-915.

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