Methods for determining the acetylation level of a peptide...

Details Methods for determining the acetylation level of a peptide... Methods for determining the acetylation level of a peptide...

2006-04-25

2006-04-25

Gitomer, Ralph (Department: 1655)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S069200

Reexamination Certificate

active

07033778

ABSTRACT:
The acetylation level of a peptide is determined utilizing the fact that the changes in the acetylation level are reflected in the sensitivity of the substrate peptide to a peptidase. This method can be used for measuring activities of deacetylase and acetylase, and also enables screening for substances that influence the activity of these enzymes. The deacetylase activity can be measured by a simple procedure according to the present invention.

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