Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-12-03
1995-06-27
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 78, 435 13, 436503, 436533, 436534, G01N 33546, G01N 33547, G01N 33567, C12Q 156
Patent
active
054279135
ABSTRACT:
The invention provides a method for determining platelet function in a mammal comprising providing platelets from the mammal, contacting the platelets in suspension with at least one immobilised ECM protein or an effective fragment or analog thereof while applying to the platelets an effective mechanical stimulus for an effective period of time and determining the platelet activation produced.
The invention also provides a method for detecting a bleeding disorder in a human. Further, the invention provides a method for monitoring the efficacy of pharmacological agents affecting platelet function in vivo.
REFERENCES:
patent: 5196309 (1993-03-01), Ginsberg
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Coller et al, 1983. Studies with a murine monoclonal antibody that abolishes ristocetin-induced binding of von Willebrand factor to platelets; additional evidence in support of GPIb as a platelet receptor for von Willbrand factor. Blood 61:99-110.
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Schwartz et al., "Insoluble Fibronectin Activates the Na/H Antiporter by Clustering and Immobilizing Integrin .alpha..sub.5 .beta..sub.1 Independent of Cell Shape", Proc. Natl. Acad. Sci. USA 88:7829-7853 (1991).
Griffith et al., "Platelet Activation by Immobilized Monoclonal Antibody: Evidence for a CD9 Proximal Signal", Blood 78:1753-1759 (1991).
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Shaw Andrew R. E.
Stewart Michael W.
Alberta Cancer Board
Grun James L.
Saunders David
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