Methods for determining phosphoryltransferase activity using...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

Reexamination Certificate

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C435S008000, C435S021000

Reexamination Certificate

active

11110435

ABSTRACT:
This invention relates to screening assay methods that can be applied to broad members of the protein kinase gene family and are useful in the detection and evaluation of kinase inhibitors.

REFERENCES:
patent: 6599711 (2003-07-01), Crouch et al.
patent: 2002/0172991 (2002-11-01), Crouch et al.
patent: 1233060 (2002-08-01), None
Kashem M et al. New Tools For Screening Protein Kinases. The Society for Biomolecular Screening 9th Annual Conference. Sep. 21-25, 2003, Portland Oregon.
Jeong S. et al. Kinase Assay Based on Thiophosphorylation of Biotinylation. BioTechniques 27(6)1232-8, Dec. 1999.
Chen, et al; Kinetic mechanism of the p38-alpha MAP kinase: phosphoryl transfer to synthetic peptides; Biochemistry; Feb. 29, 2000; vol. 39, No. 8; pp. 2079-2087.
Ward, et al; The intrinsic ATPase activity of protein kinase C is catalyzed at the active site of the enzyme; Biochemistry; Jun. 30, 1992; vol. 31, No. 25; pp. 5905-5911.
Dipolo, et al; In squid axons, ATP modulates Na+-Ca2+ exchange by a Ca2+i-dependent phosphorylation; Biochimica et Biophysica Acta; Mar. 12, 1987; vol. 897, No. 3; pp. 347-354.
Porrello, et al; Regulation of reactivated contraction in teleost retinal cone models by calcium and cyclic adenosine monophosphate; The Journal of Cell Biology; Jun. 1984; vol. 98, No. 6; pp. 2230-2238.
Cassidy, et al; Irreversible thiophosphorylation and activation of tension in functionally skinned rabbit ileum strips by [35S]ATP gamma S; The Journal of Biological Chemistry; Nov. 10, 1979; vol. 254, No. 21; pp. 11148-11153.
International Search Report for PCT/US2006/014860, 2006.

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