Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2011-01-11
2011-01-11
Lam, Ann Y (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
Reexamination Certificate
active
07867718
ABSTRACT:
The invention relates to methods for measuring conformational changes and self-assembly/aggregation of proteins, especially the formation of amyloid fibrils, using conjugated polyelectrolytes. The conjugate polyelectrolyte is exposed to the protein whereby the conjugated polyelectrolyte and the protein of interest interact, and a change of a property of the polyelectrolyte in response to conformational changes of the protein is observed. The detected change is used to determine different conformations of the protein, especially the formation of amyloid fibrils.
REFERENCES:
patent: 5545531 (1996-08-01), Rava et al.
patent: 5985242 (1999-11-01), Findeis et al.
patent: 6743640 (2004-06-01), Whitten et al.
patent: WO 9967423 (1999-12-01), None
patent: WO 02/081735 (2002-10-01), None
patent: WO 03/096016 (2003-11-01), None
Ho et al., “Optical sensors Based on Hybrid Aptamer/Conjugated Polymer Complexes”, J. Am. Chem. Soc. 2004, 126, 1384-1387.
Faid et al., “Responsive Supramolecular Polythiophene Assemblies”, J. Am. Chem. Soc. 1998, 120, 5274-5278.
Nilsson et al., [Self-assembly of synthetic peptides control conformation and optical properties of a zwitterionic polythiophene derivative, PNAS, Sep. 2, 2003, vol. 100, No. 18].
Common Core Structure of Amyloid Fibrils by Synchrotron X-ray Diffraction; Margaret Sunde et al.; pp. 729-739; Journal of Molecular Biology, 1997.
Optical Emission of a Conjugated Polyelectrolyte: Calcium-Induced Conformational Changes in Calmodulin and Calmodulin-Calcineurin Interactions; K. Peter R. Nilsson et al., pp. 9109-9113; Macromolecules, American Chemical Society; Oct. 2004.
Chip and Solution Detection of DNA Hybridization Using a Luminescent Zwitterionic Polythiophene Derivative; K. Peter R. Nilsson et al.; pp. 419-425; Nature Publishing Group, vol. 2, Jun. 2003.
Colorimetric and Fluorometric Detection of Nucleic Acids Using Cationic Polythiophene Derivatives; Hoang-Anh Ho et al.; pp. 1548-1551; Agnew. Chem. Int., 41, 9; 2002.
Optical Sensors Based on Hybrid Aptamer/Conjuqated Polymer Complexes; Hoang-Anh Ho et al.; pp. 1384-1387, JACS; Jan. 2004.
Fluorescent Polymeric Transducer for the Rapid, Simple, and Specific Detection of Nucleic Acids at the Zeptomole Level; Kim Dore et al.; pp. 4240-4244; JACS; Oct. 2003.
Polythiophene with a Free Amino Acid Side Chain; Mats Andersson et al.; pp. 546-548; Polymer Communications, 1991, vol. 32, No. 18.
Controlling Inter-chain and Intra-chain Excitations of a Poly(thiophene)Derivative in Thin Films; Magnus Berggren et al.; pp. 84-90; Chemical Physics Letters; Apr. 1999.
Photovoltaic Cells with a Conjugated Polyelectrolyte; L. Ding et al.; pp. 133-140; Synthetic Metals; Oct. 1999.
Conformational Transitions of a Free Amino-Acid-Functionalized Polythiophene Induced by Different Buffer Systems; K.P.R. Nilsson et al.; pp. 10011-10020; Journal of Physics: Condensed Matter; 2002.
Self-Assembly of Synthetic Peptides Control Conformation and Optical Properties of a Zwitterionic Polythiophene Derivative; K.Peter R. Nilsson et al. pp. 10170-10174; PNAS; Dec. 2006.
From the Cover: Twisting Macromolecular Chains: Self-Assembly of a Chiral Supermolecule from Nonchiral Polythiophene Polyanions and Random-Coil Synthetic Peptides; K. Peter R. Nilsson et al.; pp. 11197-11202; PNAS; Dec. 2006.
Asberg Peter
Hammarstrom Per
Herland Anna
Inganas Olle
Nilsson Peter
BioChromix AB
Foley & Lardner LLP
Lam Ann Y
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