Methods for determining anti-phospholipid and anti-cardiolipin a

Details Methods for determining anti-phospholipid and anti-cardiolipin a Methods for determining anti-phospholipid and anti-cardiolipin a

1991-12-06

1994-09-06

Kepplinger, Esther L.

Dentistry

Orthodontics

Tool

435 792, 435188, 435810, 436518, 436506, 436 71, 436 87, 530359, 530380, C07K 1514, G01N 3392, G01N 33543, G01N 33564

Patent

active

053447581

ABSTRACT:
A method for determining whether antiphospholipid antibodies are present in a sample is disclosed. The method comprises contacting the sample with a negatively charged phospholipid and with .beta.-2-glycoprotein-I or a homolog or analog thereof and determining whether any antiphospholipid antibodies have bound to the contacted phospholipid and .beta.-2-glycoprotein-I, wherein detection of binding of antiphospholipid antibodies to the phospholipid and .beta.-2-glycoprotein-I, is indicative that antiphospholipid antibodies are present in the sample. Also disclosed is a method for determining whether a negatively charged phospholipid is present in a sample, a kit for use in an assay for determining whether antiphospholipid antibodies are present in a sample, a kit for use in an assay for determining whether a negatively charged phospholipid is present in a sample, a method for determining whether antiphospholipid antibodies in a sample are antibodies to an autoimmune disease or antibodies to an infectious disease and a method for determining whether antiphospholipid antibodies to an autoimmune disease and antiphospholipid antibodies to an infectious disease are present in a sample.

REFERENCES:
Nimpf et al. "Prothrombinase activity of human platelets is inhibited by Pzglycoprotein-I" 1986 Biochimica et Biophysica Acta 884:142-149.
E. Politz, Protides in Biological Fluids, 27, 817-820, (1980).
M. Galli et al., LANCET, 335(8705) 1544-1547, (1990).
Murakami, H., "Keio J Med", 36(3) 284-297 (1987).
Cheng, Hwee-Ming et al., "Immunological Investigations", 18(9&10) 1121-1127 (1989).
McNeil, H. P., et al. "British Journal of Haematology", 73(4) 506-513 (1989).
Loizou, S., et al. "Clin. exp. Immunol.", 80(2) 171-176 (1990).
McNeil, H. P., et al. "Thrombosis Research", 52(6) 609-619 (1988).
Hazeltine, M. et al. "The Journal of Rheumatology", 52(1) 80-86 (1988).
McNeil, H. P. et al. "Proc. Natl. Acad. Sci. USA", 87(11) 4120-4124 (Jan. 1990).

Affiliated with

Also associated with

Rating

Methods for determining anti-phospholipid and anti-cardiolipin a does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for determining anti-phospholipid and anti-cardiolipin a, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for determining anti-phospholipid and anti-cardiolipin a will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1329163