Methods for determing cholesterol oxidase activity in an aqueous

Details Methods for determing cholesterol oxidase activity in an aqueous Methods for determing cholesterol oxidase activity in an aqueous

1995-03-09

1997-01-28

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 11, 436172, C12Q 126, C12Q 160

Patent

active

055977030

ABSTRACT:
The invention provides a method for measuring the activity of cholesterol oxidase. First, a monolayer including sterol, a phospholipid and fluorescent dye labeled phospholipid is formed on a surface of a cholesterol oxidase aqueous solution. Then, it is observed whether sterol domain fade-out occurs in the monolayer. The activity of the cholesterol oxidase can be determined on the basis of both the period of time during which the sterol domain fade-out has occurred and a concentration of sterol in a cholesterol oxidase aqueous solution. This process can be applied to a method for estimating the toxicity of chemical compounds.

REFERENCES:
patent: 4195126 (1980-03-01), Hall
Slotte, J. P., Effect of Sterol Side-Chain Structure on Sterol-Phosphatidylcholine Interactions in Monolayers and Small Unilamellar Vesicles, Biochimica et Biphysica Acta 1190 Mar. 1994 435-443.
Mattjus, P., Monolayer Interaction of Cholesterol with Phosphatidylcholines: Effects of Phospholipid Acyl Chain Length, Chemistry and Physics of Lipids, 74 Dec. 1994, 195-203.
Methods of Enzymatic Anaysis, vol. 1, Fundamentals, 3rd Edition, 1983, pp. 210-217.

Affiliated with

Also associated with

Rating

Methods for determing cholesterol oxidase activity in an aqueous does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for determing cholesterol oxidase activity in an aqueous, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for determing cholesterol oxidase activity in an aqueous will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-940803