Methods for detection of mutagens using luminescence gene

Details Methods for detection of mutagens using luminescence gene Methods for detection of mutagens using luminescence gene

1994-10-21

1997-12-30

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 6, 435 8, 536 231, 536 232, 536 237, C12Q 168, C12Q 166, C07H 2104

Patent

active

057028834

ABSTRACT:
A method for detecting or quantitating a mutagenic substance in a sample includes culturing a host microorganism transformed with a recombinant gene comprising an SOS gene and genes expressing luciferase activity and optionally genes expressing an enzyme which catalyzes the production of a substrate for luciferase, positioned downstream of the SOS gene, in a medium to which the sample is added; and measuring a luminescence generated by expression of the gene expressing luciferase activity. The method is sensitive, accurate and non-time consuming; and gene systems used for said method, i.e., a recombinant gene comprising an SOS gene expressed when a DNA is damaged and a gene expressing luciferase activity positioned downstream of the SOS gene, and a host microorganism transformed with said recombinant gene. Preferably the recombinant gene further comprises a gene expressing an enzyme which catalyses the production of a substrate for the luciferase in the down stream of the SOS gene.

REFERENCES:
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Lee et al., "Bioluminescence detection system of mutagen using firefly luciferase genes introduced in Escherichia coli lysogenic strain", Anal. Chem. 64: 1755-1759, 1992.
Mutation Research, vol. 272, 1992, pp. 91-99, Yoshimitsu Oda, et al., "A Sensitive UMU Test System For The Detection Of Mutagenic Nitroarenes In Salmonella Typhimurium NM1011 Having A High Nitroreductase Activity".
Mutation Research, vol. 147, 1985, pp. 219-229, Yoshimitsu Oda, et al., "Evaluation Of The New System (UMU-Test) For The Detection Of Environmental Mutagens And Carcinogens".
Proc. Natl. Acad. Sci. USA, vol. 79, pp. 5971-5975, Oct. 1982, Philippe Quillardet, et al. "Sos Chromotest, A Direct Assay Of Induction Of An SOS Function In Escherichia coli K-12 To Measure Genotoxicity".

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