Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-03-13
2007-03-13
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C536S024300
Reexamination Certificate
active
10197026
ABSTRACT:
The invention relates to generating a signal indicative of a target nucleic acid sequence, comprising forming a complex by incubating a sample comprising a target nucleic acid sequence with a probe comprising a first and second subunit, and a binding moiety, and dissociating the first and second subunit to release the first subunit and generate a signal. The invention also relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising forming a complex by incubating a target nucleic acid sequence, an upstream primer and a probe comprising a first and second subunit, and a binding moiety. The primer is extended with a nucleic acid polymerase to displace a portion of the first subunit from the target nucleic acid strand thereby dissociating the first subunit from the second subunit to release the first subunit and generate a signal.
REFERENCES:
patent: 4752566 (1988-06-01), Collins et al.
patent: 4766062 (1988-08-01), Diamond et al.
patent: 4818680 (1989-04-01), Collins et al.
patent: 5935791 (1999-08-01), Nadeau et al.
patent: 6350580 (2002-02-01), Sorge
patent: 6528254 (2003-03-01), Sorge
patent: 6548250 (2003-04-01), Sorge
patent: 6589743 (2003-07-01), Sorge
The Stratagene Catalog, 1988, p. 39.
Preliminary Examination Report of International Application No. PCT/US02/22722.
Lyamichev, V. et al., (1999), “Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes”,Nature Biotechnology, 17:292-296.
Tyagi, S. et al., (1996), “Molecular Beacons: Probes that Fluoresce upon Hybridization”,Nature Biotechnology, 14:303-308.
Perkin Elmer Cetus, (1988), “GeneAmp™ DNA Amplification Reagent Kit”, Part No. N801-0043, Lot No. GK 3081.
International Search Report of International Application No. PCT/US02/22722.
Sorge Joseph
Whalen Anne M.
Edwards Angell Palmer & & Dodge LLP
Horlick Kenneth R.
Spar Elizabeth N.
Stratagene California
Williams Kathleen M.
LandOfFree
Methods for detection of a target nucleic acid by capture... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for detection of a target nucleic acid by capture..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for detection of a target nucleic acid by capture... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3804075