Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...
Reexamination Certificate
2011-01-18
2011-01-18
Therkorn, Ernest G (Department: 1797)
Liquid purification or separation
Processes
Liquid/liquid solvent or colloidal extraction or diffusing...
C210S656000, C210S198200, C073S061520, C435S006120, C536S025400
Reexamination Certificate
active
07871519
ABSTRACT:
Provided is a method comprising introducing a sample comprising a plurality of oligonucleotides into an ion pair high performance liquid chromatography column having a buffered mobile phase and allowing at least a portion of the oligonucleotides to separate; allowing the oligonucleotides to elute from the column; and introducing the oligonucleotides into a mass spectrometer and quantifying at least a portion of the oligonucleotides by mass spectrometry. In the method, at least a portion of the oligonucleotides are co-eluting oligonucleotides that differ in mass by no more than 20%; and the buffered mobile phase causes at least 50 mole percent of co-eluting oligonucleotides to have the same charge when they enter the mass spectrometer.
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Capaldi Daniel
Gaus Hans-joachim
Kurata Christine
Luu Nhuy
Rentel Claus André Frank
ISIS Pharmaceuticals Inc.
Therkorn Ernest G
Woodcock & Washburn LLP
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