Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-03-05
1998-03-03
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 792, 536 243, 536 2432, 536 2532, 935 77, 935 78, C12Q 168, C07H 2104, C07H 2102
Patent
active
057232946
ABSTRACT:
The present invention provides methods and apparatus for detecting and discriminating multiple analytes within a test sample which are rapid, accurate, and convenient enough for routine use in a clinical laboratory. In particular, the present invention provides methods and apparatus for permitting multiple PCR-amplified target nucleic acid sequence hybrids within a single sample, labeled with different fluorescent dyes, to be spectrally distinguished using the read-out data directly from a fluorescence reader instrument.
REFERENCES:
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5424841 (1995-06-01), Van Gelder et al.
patent: 5453355 (1995-09-01), Birkenmeyer et al.
patent: 5459034 (1995-10-01), Tabaqchali et al.
Iitia, Antti et al. "Simultaneous Detection of Two Cystic Fibrosis Alleles Using Dual-Label Time-Resolved Fluorometry,"Molecular and Cellular Probes, 1992, vol. 6, pp. 505-511. (whole document).
DeBiasio, R., Bright, G.R., Ernst, L.A., Waggoner, A.S., Taylor, D.L., Five-Parameter Fluorescence Imaging: Wound Healing of Living Swiss 3T3 Cells, J. Cell Biology, 1987; 105: 1613-1622.
Ried, T., Baldini, A., Rand, T.C., Ward, D.C., Simultaneous Visualization of Seven Different DNA Probes by in situ Hybridization Using Combinatorial Fluorescence and Digital Imaging Microscopy, Proc. Natl. Acad. Sci. USA, 1992; 89: 1388-1392.
Iovannisci, D.M., Winn-Deen, E.S., Ligation Amplification and Fluorescence Detection of Mycobacterium tuberculosis DNA, Molecular and Cellular Probes, 1993; 7:35-43.
Vlieger, A.M., Medenblik, A.M.J.C., VanGijswijk, P.M., Tanke, H.J., Van Der Ploeg, M., Gratama, J.W., Raap, A.K., Quantitation of Polymerase Chain Reaction Products by Hybridization-Based Assays with Fluorescent, Colorimetric, or Chemiluminescent Detection, Analytical Biochemistry, 1992; 205:1-7.
Gudibande, S.R., Kenten, J.H., Link, J., Friedman, K. Massey, R. J., Rapid, Non-Separation Electrochemiluminescent DNA Hybridization Assays for PCR Products, Using 3'-Labelled Oligonucleotide Probes, Molecular and Cellular Probes, 1992, 6:495-503.
Wu, L., McCarthy, B.J., Kadushin, J.M., Nuss, C.E., A Simple and Economic Method for Directly Performing PCR on Washed Blood Cells or on Whole Blood, Transgenica, 1994: 1(1): 1-5.
Wu, L., Chaar, O., Bradley, K., Kadushin, J., HLA DR DNA Typing With a Microtiter Plate-Based Hybridization Assay, Hum. Immun. 1993; 37: p. 141.
Bauer, H., Ting., Y., Greer, C., Chambers, J., Tashiro, C., Chimera, J., Reingold, A., Monos, M., JAMA 265:472-477(1991), Genital Human Papillomavirus Infection in Female University Students and Determined by a PCT-Based Method.
Nuijten, P., Van Asten, F., Gaastra, W., Van Der Zeijst, B., J. Biol. Chem. 265:17798-17804 (1990), "Structural and Functional Analysis of Two Campylobacter jejuni Flagellin Genes".
Stone, G., Oberst, R., Hays, M., McVey, S., Chengappa, M., J. Clin. Miocrobiol. 32: 1742-1749 (1994), Detection of Salmonella Serovars from Clinical Samples by Enrichment Broth Cultivation-PCR Precedure.
Galan, J., Ginocchio, C., Costeas, P., J. Bacterial, 174:4338-4348 (1992), Molecular and Functional Characterization of the Salmonella Invasion Gene invA; Homology of InvA to Members of a New Protein Family.
Sakai, T., Saskawa, C., Makiro, S., Yoshikawa, M., Infect. Immun. 54:395-402 (1986), DNA Sequence and Product Analysis of the virF Locus Responsible for Congo Red Binding and Cell Invasion in Shigella flexneri 2a.
Yavzori, M., Cohen, D., Wasserlauf, R., Ambar, R., Rechavi, G., Ashkenazi, S., Eur. J. Clin. Microbiol. Infect. Dis. 13:232-237 (1994), Identification of Shigella Species in Stool Specimens by DNA Amplification of Different Loci of the Shigella Virulence Plasmid.
Yi-Kun, L., Qin, H., Molodysky, E., Morris, B., J. Virol. Methods 44L77-78 (1994), Simple Microwave and Thermal Cycler Boiling Methods for Preparation of Cervicovaginal Lavage Cell Samples Prior to PCR For Human Papillomavirus Detection.
Ohhara, M., Kursou, Y., Esumi, M., Biotechniques 17:726-728 (1994), Direct PCR of Whole Blood and Hair Shafts by Microwave Treatment.
Singh, R.K., Tell, S.G., White, C.T., Hoffman, D., Chi, V.L. & Erickson, B.W. (1993), A Scalable Systolic Multiprocessor System for Analysis of Biological Sequences. Research on Integrated Systems: Proceeding of the 1993 Symposium, MIT Press, Cambridge, MA, 168-182.
Cebula, T., Payne, W., Feng, P. J. Clin. Microbiol. 33:248-250 (1995), Simultaneous Identification of Strains of Escherichia coli Serotype O157:H7 and Their Shiga-Like Toxin Type of Mismatch Amplification Mutation Assay-Multiplex PCR.
Nakajima, H., Inoue, M., Mori, T., Itoh, K., Arakawa, E., Watanabe, H., J. Clin Microbiol. 30:2484-2486 (1992), Detection and Identification of Yersinia pseudotuberculosis and Pathogenic Yersinia enterocolitica by Improved Polymerase Chain Reaction Method.
Wegmuller, B., Luthy, Jr. Candrian, U., Applied and Environmental Microbiology, 59:2161-2165 (1993), Direct Polymerase Chain Reaction Detection of Camylobacter jejuni and Campylobacter coli in Raw Milk and Dairy Products.
Chamberlain, J., Gibbs, R., Ranier, J., Caskey, C., PCR Protocols: A Guide to Methods and Applications. pp. 272-281.
Coombs Jana
Glass Michael J.
Malmstrom Sharon L.
Wu Linxian
Gull Laboratories
Jones W. Gary
Tran Paul B.
LandOfFree
Methods for detection and discrimination of multiple analytes us does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for detection and discrimination of multiple analytes us, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for detection and discrimination of multiple analytes us will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2246641