Methods for detecting thiol-containing nucleotide...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C435S015000, C435S021000

Reexamination Certificate

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07807399

ABSTRACT:
Disclosed are methods for detecting thiol-containing nucleotide diphosphates. The methods utilize thiol-reactive fluorescent reagents.

REFERENCES:
patent: 7585643 (2009-09-01), Sem
patent: 2007/0054410 (2007-03-01), Sem et al.
Cohen, Philip, “The Origins of Protein Phosphorylation”, Nature Cell Biology, vol. 4, May 2002, pp. E127-E130.
Cohen, Philip, “The Role of Protein Phosphorylation in Human Health and Disease”, The Sir Hans Krebs Medal Lecture, Delivered on Jun. 30, 2001 at the FEBS Meeting in Lisbon, MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Scotland; Eur. J. Biochem 268, pp. 5001-5010 (2001).
Hanks, Steven K. and Hunter, Tony, “The Eukaryotic Protein Kinase Superfamily: Kinase (Catalytic) Domain Structure and Classification”, Protein Kinases 6, The FASEB Journal, vol. 9, May 1995, pp. 576-596.
Marras, Salvatore A. E., Kramer, Fred Russell, and Tyagi, Sanjay, “Efficiencies of Fluorescence Resonance Energy Transfer and Contact-Mediated Quenching in Oligonucleotide Probes”, Nucleic Acids Research, 2002, vol. 30, No. 21, e122, pp. 1-8.
Nick, Jerry A. et al, “Role of p38 Mitogen-Activated Protein Kinase in a Murine Model of Pulmonary Inflammation”, The Journal of Immunology, 2000, 164: pp. 2151-2159.
Zaman, G. J. R., Garritsen, A., De Boer, TH., and Van Boeckel C. A. A., “Fluorescence Assays for High-Throughput Screening of Protein Kinases”, Combinatorial Chemistry & High Throughput Screening, 2003, vol. 6, No. 4, pp. 313-320.
Xia, Wensheng et al, “Applications of Fluorescent Polymer Superquenching to High Throughput Screening Assays for Protein Kinases”, Assay and Drug Development Technologies, vol. 2, No. 2, 2004, pp. 183-192.
Blume-Jensen, Peter and Hunter, Tony, “Oncogenic Kinase Signalling”, Nature, vol. 411, May 17, 2001, pp. 355-365.
Manning, G., Whyte, D. B., Martinez, R., Hunter, T., and Sudarsanam, S., “The Protein Kinase Complement of the Human Genome”, Science, Dec. 6, 2002, vol. 298, pp. 1912-1916, 1933-1934.
Daly, Thomas J., Olson, John S., and Matthews, Kathleen Shive, “Formation of Mixed Disulfide Adducts at Cysteine-281 of the Lactose Repressor Protein Affects Operator and Inducer Binding Parameters”, Biochemistry, 1986, vol. 25, pp. 5468-5474.
Ways, D. Kirk, and Sheetz, Matthew J., “The Role of Protein Kinase C in the Development of the Complications of Diabetes”, Vitamins and Hormones, vol. 60, pp. 149-193, Copyright © 2001.
Eckstein, Fritz and Goody, Roger S., “Synthesis and Properties of Diastereoisomers of Adenosine 5′-(O-1-Thiotriphosphate) and Adenosine 5′-(O-2-Thiotriphosphate)” Biochemistry, vol. 15, No. 8, 1976, pp. 1685-1691.
Viola, Ronald E., Raushel, Frank M., Rendina, Alan R., and Cleland, W. W., “Substrate Synergism and the Kinetic Mechanism of Yeast Hexokinase”, Biochemistry, 1982, vol. 21, pp. 1295-1302.
Pullela, Phani Kumar, Chiku, Taurai, and Sem, Daniel S., “Fluorescence Probes with Utility in Kinase Assay and Redox Sensing”, The FASEB Journal, Mar. 4, 2005, vol. 19, No. 4, Experimental Biology/IUPS 2005: Meeting Abstracts, p. A265.
Invitrogen Product Description for BODIPY® FL L-cystine available at least as early as Jan. 1, 2003.
Chiku, Taurai, Pullela, Phani Kumar, and Sem, Daniel S., “A Dithio-Coupled Kinase and ATPase Assay”, Journal of Biomolecular Screening (X)X; 2006, pp. 1-10.
Chiku, Taurai, Pullela, Phani Kumar, and Sem, Daniel S., “A General Dithiol-Coupled UV Vis and Fluorescence Assay for Kinases”, FASEB Journal, 19 (4, Part 1 Suppl.) A265-266, Abstract 212.4, 2005.
Churchich, Jorge E. and Wu, Christine, “Nucleoside Phosphorothioates As Probes of the Nucleotide Binding Site of Brain Pyridoxal Kinase”, The Journal of Biological Chemistry, vol. 257, No. 20, Oct. 25, 1982, pp. 12136-12140.
Kupcho, Kevin, Somberg, Richard, Bulleit, Bob, and Goueli, Said A., “A Homogeneous, Nonradioactive High-Throughput Fluorogenic Protein Kinase Assay”, Analytical Biochemistry, 317 (2003) pp. 210-217.
Pullela, Phani Kumar, Chiku, Taurai, Carvan, Michael J. (III), and Sem, Daniel S., “Fluorescence-Based Detection of Thiols in Vitro and in Vivo Using Dithiol Probes”, Analytical Biochemistry, 352 (2006) pp. 265-273.

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