Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Reexamination Certificate
2006-11-07
2006-11-07
Gitomer, Ralph (Department: 1655)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
C424S009100
Reexamination Certificate
active
07132252
ABSTRACT:
The invention provides methods of using an optical molecular probe or sensor to screen candidate drugs for their interaction with at least one cytochrome P450 enzyme. The optical molecular probe useful in the methods of the present invention is a compound having the generic structure Y-L-Q, wherein Y is selected from the group consisting of Q as herein defined, saturated C1–C20alkyl, unsaturated C1–C20alkenyl, unsaturated C1–C20alkynyl, substituted saturated C1–C20alkyl, substituted unsaturated C1–C20alkenyl, substituted unsaturated C1–C20alkynyl, C1–C20cycloalkyl, C1–C20cycloalkenyl, substituted saturated C1–C20cycloalkyl, substituted unsaturated C1–C20cycloalkenyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl; L is selected from the group of (—OCR2H)p—, wherein for each p, all R2are separately selected from the group consisting of a hydrogen atom, saturated C1–C20alkyl, unsaturated C1–C20alkenyl, unsaturated C1–C20alkynyl, substituted saturated C1–C20alkyl, substituted unsaturated C1–C20alkenyl, substituted unsaturated C1–C20alkynyl, C1–C20cycloalkyl, C1–C20cycloalkenyl, substituted saturated C1–C20cycloalkyl, substituted unsaturated C1–C20cycloalkenyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, and p is a positive integer no greater than twelve; and Q is a chemical moiety that gives rise to optical properties in its hydroxy or hyrdoxylate, phenol or phenoxide form that are different from the optical properties that arise from its ether form. Most preferably, p is one, R2is hydrogen, and Q is the ether form of a phenoxide fluorophore.
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Makings Lewis R.
Zlokarnik Gregor
Gitomer Ralph
Invitrogen Corporation
Sterne Kessler Goldstein & Fox P.L.L.C.
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