Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1999-01-12
2000-07-04
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
424 934, 4242821, 435 29, 435 34, 4352521, 435822, 435975, C12Q 100
Patent
active
060836830
ABSTRACT:
This invention relates generally to in vitro methods for inducing or enhancing expression of enteric bacterial antigens and/or virulence factors thereby producing antigenically enhanced enteric bacteria, to methods for using antigenically enhanced enteric bacteria and to vaccines comprising antigenically enhanced enteric bacteria. A Shigella bacterium having enhanced antigenic property is disclosed. A culture medium useful for culturing the Shigella comprises 0.05% to 3% bile or 0.025% to 0.6% of one or more bile acids or salts. In addition a divalent cation chelator can also be included in the culture medium. The bacteria culture is in a growth phase at early log phase and between early log phase and stationary phase. The enhanced antigenic property is a higher level of an immunogenic antigen when compared to the antigenic property of the same bacteria grown on brain heart infusion broth. Also, antibodies to Shigella bacteria or Shigella bacteria in samples are detected by immunoassays.
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Frey Steven Michael
Pace John Lee
Walker Richard Ives
Antex Biologics Inc.
Naff David M.
Ware Deborah K.
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