Methods for detecting polypeptides regulating signal...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S320100

Reexamination Certificate

active

07087382

ABSTRACT:
A method of detecting a polypeptide capable of regulating a transduction pathway and an expression library for use with such method are provided. The method is effected by introducing into cells endogenously expressing a trans acting regulator of the transduction pathway the expression library which includes a plurality of expression constructs each encoding a polypeptide and a reporter molecule expressible in the presence of a trans acting regulator of the transduction pathway. A level of expression within a predetermined range of the reporter is indicative of regulation of the transduction pathway by the polypeptide.

REFERENCES:
patent: 5482835 (1996-01-01), King et al.
patent: 5753446 (1998-05-01), Johnson
patent: 5876972 (1999-03-01), Spengler et al.

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