Methods for detecting pancreatic beta-islet cells and...

Drug – bio-affecting and body treating compositions – In vivo diagnosis or in vivo testing – Magnetic imaging agent

Reexamination Certificate

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C424S009300, C424S009323, C424S009600, C424S009341, C530S387300, C530S387900, C530S388100, C530S387100, C530S388150, C435S007100, C435S007200, C435S007210, C435S007230

Reexamination Certificate

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07906104

ABSTRACT:
The role of synaptic adhesion molecules in human cell development and function is largely unknown. This invention provides methods to study β-cell function in native tissue through the use of novel adhesion and migrations assays. Through the use of these assays, the inventors have been able to for the first time describe the contribution of SAMs to human β-cell adhesion, spreading, and motility. Furthermore, the inventors have used the results of these assays to develop methods for detection, treatment, and prevention of diseases related to the pancreas.

REFERENCES:
Fujita E et al. Distribution of RA175/TSLC1/SynCAM, a member of the immunoglobulin superfamily, in the developing nervous system. Feb. 2005; 154(2):199-209.
Bolliger MF et al. Identification of a novel neuroligin in humans which binds to PSD-95 and has a widespread expression. Biochem J. 2001; 356:581-588.
Song J-Y et al. Neuroligin 1 is a postsynaptic cell-adhesion molecule of excitatory synapses. Proc. Natl. Acad. Sci. USA, 1999; 96:1100-1105.
X-linked neuroligin 4 [Homo sapiens] protein sequence. NCBI reference sequence NP—065793.1. Accessed on Dec. 3, 2009.
Fukahi K et al. Aberrant expression of Neuropilin-1 and -2 in human pancreatic cancer cells. Clin Cancer Res. Jan. 2004; 10:581-590.
Parikh AA et al. Expression and regulation of the novel vascular endothelial growth factor receptor Neuropilin-1 by epidermal growth factor in human pancreatic carcinoma. Cancer, 2003; 98:720-729.
Suckow AT et al. Expression of Neurexin, Neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of Neuroligin in insulin secretion. Endocrinology. 2008; 148:6006-6017.

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