Methods for detecting glucagon antagonists

Details Methods for detecting glucagon antagonists Methods for detecting glucagon antagonists

1991-08-08

1995-04-18

Wax, Robert A.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Glucagon; related peptides

A61K 3700, C07K 700

Patent

active

054080371

ABSTRACT:
Methods for detecting glucagon antagonists through the use of recombinant DNA techniques are provided. Briefly, subsequent to the expression of glucagon analogs within suitable host cells, the analogs are exposed to a glucagon receptor coupled to a response pathway in the presence of native glucagon. A reduction in the stimulation of the response pathway resulting from the binding of the glucagon analog to the glucagon receptor relative to the stimulation of the response pathway by native glucagon alone indicates the presence of a glucagon antagonist. Glucagon antagonists identified and isolated through the methods are also provided.

REFERENCES:
Zuranski et al EMBO vol. 8 No. 9 pp. 2583-2590 Sep. 1989.
Gysin et al. Biochemistry vol. 25 pp. 8278-8284, 1986.
Merrifield, R. B. et al., "Synthetic Peptide Antagonists of Glucagon," in Theodoropoulos (ed.), Peptides 1986. Proceedings of the 19th European Peptide Symposium (Chilkidiki, Greece) Walter de Gruyte, New York, 1987, pp. 517-520.

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