Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-05-15
2007-05-15
Rawlings, Stephen L. (Department: 1643)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C530S350000, C530S387900, C530S388850, C530S389100, C530S387100, C530S387300, C530S391300
Reexamination Certificate
active
10276312
ABSTRACT:
A method for detecting DNA damage in a tissue sample involves contacting an immobilized biological sample with a labeled ligand which binds to human 53Bp1, and examining the immobilized sample for the presence of a label generated-detectable signal concentrated in foci in said sample. The presence of concentrated foci is indicative of DNA damage and the presence of diffuse signal is indicative of a normal sample. Diagnostic reagents contain a ligand that binds to human 53Bp1 associated with a detectable label. Diagnostic kits for detecting DNA damage in a biological sample contain such diagnostic reagents and signal detection components. Compositions that inhibit or antagonize the biological activity of 53Bp1 are identified by suitable assays, and are employed in methods of retarding the growth of a cancer cell.
REFERENCES:
Rappold et al. (J. Cell Biol. Apr. 30, 2001; 153 (3): 613-20).
Skolnick et al. (Trends in Biotechnology. 2000; 18 (1): 34-39).
Wu et al. (J. Immunol. 2005; 174: 934-941).
Tuteja et al. (Crit. Rev. Biochem. Mol. Biol. 2001; 36 (6): 261-290).
DiTullio et al. (Nature Cell Biol. Dec. 2002; 4: 998-1002; provided with supplementary information and corrigendum).
Jullien et al. (J. Cell Sci. 2002; 115: 71-79).
Mariuzza et al. (Annu. Rev. Biophys. Biophys. Chem. 1987; 16: 139-159).
Gussow et al. (Methods in Enzymology. 1991; 203: 99-121).
Giusti et al. (Proc. Natl. Acad. Sci. USA. May 1987; 84 (9): 2926-2930).
Chien et al. (Proc. Natl. Acad. Sci. USA. Jul. 1989; 86 (14): 5532-5536).
Caldas et al. (Mol. Immunol. May 2003; 39 (15): 941-952).
J.H. Petrini, “DNA Repair '99—The Mammalian Mre11-Rad50-Nbs1 Protein complex: Integration of Functions in the Cellular DNA-Damage Response”,Am J. Hum. Genet., 64:1264-1269 (Apr. 6, 1999).
S.J. Elledge, “Cell Cycle Checkpoints: Preventing an Identity Crisis”,Science, 274:1664-1672 (Dec. 6, 1996).
M.P. Longhese et al., “DNA Damage Checkpoint in Budding Yeast”,EMBO J., 17(19):5525-5528 (Oct. 1, 1998).
T. Weinert, “DNA Damage Checkpoints Update: Getting Molecular”,Curr. Opin. Genet. Dev., 8:185-193 (Apr. 1998).
L. Hartwell, “Defects in a Cell Cycle Checkpoint May Be Responsible for the Genomic Instability of Cancer Cells”,Cell, 71:543-546 (Nov. 13, 1992).
C. Lengauer et al., “Genetic Instabilities in Human Cancers”,Nature, 396:643-649 (Dec. 17, 1998).
L.A. Loeb, “Mutator Phenotype May Be Required for Multistage Carcinogenesis”,Cancer Res., 51:3075-3079 (Jun. 15, 1991).
T. Bessho et al., “Human DNA Damage Checkpoint Protein hRAD9 is a 3′ to 5′ Exonuclease”,J. Biol. Chem., 275:7451-7454 (Mar. 17, 2000).
H.B. Lieberman et al., “A Human Homolog of theSchizosaccharomyces pombe rad9+Checkpoint Control Gene”,Proc. Natl. Acad. Sci. USA, 93:13890-13895 (Nov. 1996).
R.P.ST. Onge et al., “The Human G2 Checkpoint Control Protein hRAD9 is a Nuclear Phosphoprotein that Forms complexes with hRAD1 and hHUS1”,Mol. Biol. Cell., 10:1985-1995 (Jun. 1999).
E. Volkmer et al., “Human Homologs ofSchizosaccharomyces pombeRad1, Hus1, and Rad9 Form a DNA Damage-Responsive Protein Complexes”,J. Biol. Chem., 274:567-570 (Jan. 8, 1999).
K. Savitsky et al., “A Single Ataxia Telangiectasia Gene with a Product Similar to PI-3 Kinase”,Science, 268:1749-1753 (Jun. 23, 1995).
N.J. Bentley et al., “TheSchizosaccharomyces pombe rad 3Checkpoint”,EMBO J., 15(23):6641-6651 (Dec. 2, 1996).
T.D. Halazonetis et al., “Many Faces of ATM: Eighth International Workshop on Ataxia-Telangiectasia”,Biochim. Biophys,. Acta, 1424:R45-55 (Oct. 29, 1999).
K.A. Cimprich et al., “cDNA Cloning and Gene Mapping of a Candidate Human Cell Cycle Checkpoint Protein”,Proc. Natl. Acad. Sci. USA, 93:2850-2855 (Apr. 1996).
A. Blasina et al., “A Human Homologue of the Checkpoint Kinase Cds1 Directly Inhibits Cdc25 Phosphatase”,Curr. Biol., 9:1-10 (Jan. 1999).
A.L. Brown et al., “A Human Cds1-Related Kinase that Functions Downstream of ATM Protein in the Cellular Response to DNA Damage”,Proc. Natl. Acad. Sci. USA, 96:3745-3750 (Mar. 1999).
S. Matsuoka et al., “Linkage of ATM to Cell Cycle Regulation by the Chk2 Protein Kinase”,Science, 282:1893-1897 (Dec. 4, 1998).
N.H. Chehab et al., “Chk2/hCds1 Functions as a DNA Damage Checkpoint in G1by Stabilizing p53”,Genes Dev., 14:278-288 (Feb. 1, 2000).
D.W. Bell et al., “Heterozygous Germ Line hCHK2 Mutations in Li-Fraumeni Syndrome”,Science, 286:2528-2531 (Dec. 24, 1999).
T. Weinert et al., “TheRAD9Gene Controls the Cell Cycle Response to DNA Damage inSaccharomyces cerevisiae”, Science, 241: 317-322 (Jul. 15, 1988).
P. Bork et al., “A Superfamily of Conserved Domains in DNA Damage-Responsive Cell Cycle Checkpoint Proteins”,FASEB. J., 11:68-76 (Jan. 1997).
I. Callebaut et al., “From BRCA1 to RAP1: A Widespread BRCT Module Closely Associated with DNA Repair”,FEBS Lett., 400:25-30 (Jan. 2, 1997).
A.G. Paulovich et al., “When Checkpoints Fail”,Cell, 88:315-321 (Feb. 7, 1997).
Y. Sanchez et al., “Regulation of RAD53 by theATM-Like KinasesMEC1andTEL1in Yeast Cell Cycle Checkpoint Pathways”,Science, 271:357-360 (Jan. 19, 1996).
Z. Sun et al., “Spk1/Rad53 is Regulated by Mec1-Dependent Protein Phosphorylation in DNA Replication and Damage Checkpoint Pathways”,Genes Dev., 10:395-406 (Feb. 15, 1996).
R.S. Maser et al., “hMre11 and hRad50 Nuclear Foci are Induced During the Normal Cellular Response to DNA Double-Strand Breaks”,Mol. Cell. Biol., 17:6087-6096 (Oct. 1997).
B.E. Nelms et al., “In Situ Visualization of DNA Double-Strand Break Repair in Human Fibroblasts”,Science, 280:590-592 (Apr. 24, 1998).
M. Löbrich et al., “Repair of X-Ray-Induced DNA Double-Strand Breaks in SpecificNotI Restriction Fragments in Human Fibroblasts: Joining of Correct and Incorrect Ends”,Proc. Natl. Acad. Sci. USA, 92:12050-12054 (Dec. 1995).
R. Scully et al., “Dynamic Changes of BRCA1 Subnuclear Location and Phosphorylation State are Initiated by DNA Damage”,Cell, 90:425-435 (Aug. 8, 1997).
J.S. Lee et al., “hCds1-Mediated Phosphorylation of BRCA1 Regulates the DNA Damage Response”,Nature, 404:201-204 (Mar. 9, 2000).
Y. Wang et al., “BASC, a Super Complex of BRCA1-Associated Proteins Involved in the Recognition and Repair of Aberrant DNA Structures”,Genes Dev., 14:927-939 (Apr. 15, 2000).
Q. Zhong et al., “Association of BRCA1 with the hRad50-hMre11-p95 complex and the DNA Damage Response”,Science, 285:747-750 (Jul. 30, 1999).
T. Haaf et al., “Nuclear Foci of Mammalian Rad51 Recombination Protein in Somatic Cells After DNA Damage and its Localization in Synaptonemal Complexes”,Proc. Natl. Acad. Sci. USA, 92:2298-2302 (Mar. 1995).
T.L. Tan et al., “Mouse Rad54 Affects DNA Conformation and DNA-Damage-Induced Rad51 Foci Formation”,Curr. Biol., 9(6):325-328 (Mar. 25, 1999).
C. Morrison et al., “The Controlling Role of ATM in Homologous Recombinational Repair of DNA Damage”,EMBO J., 19:463-471 (2000).
C. Morrison et al., “The Controlling Role of ATM in Homologous Recombinational Repair of DNA Damage”,EMBO J., 19(4):786 (2000).
V. Gharibyan et al., “Localization of the Bloom Syndrome Helicase to Punctate Nuclear Structures and the Nuclear Matrix and Regulation during the Cell Cycle: Comparison with the Werner's Syndrome Helicase”,Mol. Carcinog., 26:261-273 (Dec. 1999).
T. Lindahl et al., “Quality Control by DNA Repair”,Science, 286:1897-1905 (Dec. 3, 1999).
K. Iwabuchi et al., “Two Cellular Proteins that Bind to Wild-Type but not Mutant p53”,Proc. Natl. Acad. Sci. USA, 91:6098-6102 (Jun. 1994).
K. Iwabuchi et al., “Stimulation of p53-Mediated Transcriptional Activation by the p53-Binding Proteins”,J. Biol
Halazonetis Thanos
Schultz Linda B.
Howson & Howson LLP
Rawlings Stephen L.
The Wistar Institute of Anatomy and Biology
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