Methods for detecting circulating mutant BRAF DNA

Details Methods for detecting circulating mutant BRAF DNA Methods for detecting circulating mutant BRAF DNA

2006-01-24

2008-10-28

Benzion, Gary (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S091200

Reexamination Certificate

active

07442507

ABSTRACT:
The present invention relates to a method for detecting the presence of circulating mutant BRAF DNA, which may be present in circulating melanoma cells or as DNA shed from tumor cells. Methods, compositions and kits which employ one or more sets of BRAF mutant specific primer pairs for detection of circulating mutant BRAF DNA are presented. Also provided are methods for diagnosing and/or determining stage/progression of a melanoma in a mammal based on detection of a BRAF mutant nucleic acid sequence. Such methods are also well suited to monitoring disease activity in patients with active disease or those in remission.

REFERENCES:
Xu et al., High Prevalence of BRAF Gene Mutation in Papiliary Thyroid Carcinomas and Thyroid Tumor Cell Lines, Cancer Research, 63:4561-4567 (2003).
Iinuma et al., Detection of Tumor Cells in Blood Using CD45 Magnetic Cell Separation Followed By Nested Mutant Allele-Specific Amplification ofp53and K-rasGenes in Patients Colorectal Cancer, Int. J. Cancer (Pred. Oncol.), 89:337-344 (2000).
C. Esche, First Study on BRAF Mutation In the Serum of Melanoma Patients, 553, vol. 122, No. 3, Mar. 2004 (Abstract).
Yancovitz et al., Detection of MutantBRAFAlleles In The Plasma of Patients With Metastatic Melanoma, Journal of Molecular Diagnostics, 9:178-183 (2007).
Kondo et al., Detection of Point Mutations in the K-rasOncogene at Codon 12 in Pure Pancreatic Juice For Diagnosis of Pancreatic Carcinoma, Cancer, 73:1589-1594 (1994).
Pellegata et al., K-rasp53Mutations in Pancreatic Cancer: Ductal and Nonductal Tumors Progress through Different Genetic Lesions, Cancer Research, 54:1556-1560 (1994).
Yamada et al., Detection of K-rasGene Mutations in Plasma DNA of Patients with Pancreatic Adenocarcinoma: Correlation with Clinicopathological Features, Clinical Cancer Research, 4:1527-1532 (1998).
Kopreski et al., Detection of mutant K-rasDNA in plasma or serum of patients with colorectal cancer, British Journal of Cancer, 76:1293-1299 (1997).
Miller et al., The Journal of Investigative Dermatology, 123:990-992 (2004).

Affiliated with

Also associated with

Rating

Methods for detecting circulating mutant BRAF DNA does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for detecting circulating mutant BRAF DNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for detecting circulating mutant BRAF DNA will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4007529