Methods for detecting cell apoptosis

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 722, 435 721, 435 723, 435 724, 435 79, 435 792, 435 793, 435 794, 435 795, 436501, 436503, 436504, 436515, 436536, 436544, 436545, 436546, 5303879, G01N 3353, G01N 33567, G01N 33536, C07K 1600

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060487038

ABSTRACT:
Methods for the biochemical and immunohistochemical detection of cell apoptosis are described. The methods utilize the detection and measurement of polypeptide fragments generated during apoptosis. Conditions associated with apoptosis may be detected by the methods of this invention. Methods are also presented for the screening of potential therapeutic compounds which inhibit or stimulate apoptosis. Kits for detection of apoptosis and diagnosis of diseases are also provided.

REFERENCES:
patent: 5536639 (1996-07-01), Siman et al.
patent: 5605826 (1997-02-01), Wright eet al.
Amendola et al Proc. Natl. Acad. Sci. vol. 93,(20)pp. 11057-62, Oct. 1, 1996.
Catty et al Antibodies vol. II Ed by D. Catty (1989) IRL Press pp. 97-154, 1989.
Harlow et al Antibodies Ed by Harlow & Lane CSHL Press Ch 12-pp. 473-510, 1988.
Alnenui et al., "Cloning and Expression of Four Novel Isoforms of Human Interleukin-1.beta. Converting Enzyme with Different Apoptotic Activities", J. Biol. Chem., 1995, 270(9) , 4312-4317.
Batistatou et al., "Bcl-2 Affects Survival but Not Neuronal Differentiation of PC12 Cells", J. Neurosci., 1993, 13(10) , 4422-4428.
Cherney et al., "cDNA sequence, protein structure, and chromosomal location of the human gene for poly (ADP-ribose) polymerase", Proc. Natl. Acad. Sci. USA, 1987, 84, 8370-8374.
Cohen et al., "Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation", Biochem. J., 1992, 286, 331-334.
Collins et al., "Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture", Nature, 1977, 270, 347-349.
Crompton et al., "Propidium iodide staining correlates with the extent of DNA degradation in isolated nuclei", Biochem. Biophys. Res. Commun., 1992, 183(2) , 532-537.
Duan et al., "ICE-LAP6, a Novel Member of the ICE/Ced-3 Gene Family, Is Activated by the Cytotoxic T Cell Protease Granzyme B", J. Biol. Chem,, 1996, 271,(28) 16720-16724.
Enari et al., "Involvement of an ICE-lik protease in Fas-mediated apoptosis", Nature, 1995, 375, 78-81.
Faucheu et al., "A novel human protease similar to the interleukin-1.beta. converting enzyme induces apoptosis in transfected cells", EMBO J., 1995, 14(9), 1914-1922.
Enari et al., "Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosis", Nature, 1996, 380, 723-726.
Fernandes-Alnemri et al., "CPP32, a Novel Human Apoptotic Protein with Homology to Caenorhabditis elegans Cell Death Protein Ced-3 and Mammalian Interleukin-1.beta.-converting Enzyme", J. Biol. Chem., 1994, 269 (49) , 30761-30764.
Fernandes-Alnemri et al., "Mch2, a New Member of the Apoptotic Ced-3/Ice Cysteine Protease Gene Family", Cancer Res., 1995, 55, 2737-2742.
Frey, "Nucleic Acid Dyes for Detection of Apoptosis in Live Cells", Cytometry, 1995, 21, 265-274.
Gagliardini et al., "Prevention of Vertebrate Neuronal Death by the crmA Gene", Science, 1994, 263, 826-828.
Gavrieli et al., "Identification of Programmed Cell Death In Situ via Specific Labeling of Nuclear DNA Fragmentation", J. Cell Biol., 1992, 119(3) , 493-501.
Gu et al., "Cleavage of Poly(ADP-ribose) Polymerase by Interleukin-1.beta. Converting Enzyme and Its Homologs TX and Nedd-2", J. Biol. Chem., 1995, 270(32) , 18715-18718.
Hoeppner et al., "Programmed cell death: from development to disease", Biochim. Biophys. Acta, 1966, 1242, 217-220.
Huppi et al., "Sequence and organization of the mouse poly (ADP-ribose) polymerase gene", Nucl. Acids. Res., 1989, 17(9) , 3387-3401.
Ittel et al., "Chicken poly(ADP-ribose) synthetase: complete deduced amino acid sequence and comparison with mammalian enzyme sequences", Gene, 1991, 102, 157-164.
Kamens et al., "Identification and Characterization of ICH-2, a Novel Member of the Interleukin-.beta.-converting Enzyme Family of Cysteine Protease", J. Biol. Chem., 1995, 270(25), 15250-15256.
Fernandez-Alnemri et al., "Mch3, a Novel Human Apoptotic Cysteine Protease Highly Related to CCP32", Cancer Res., 1995, 55, 6045-6052.
Kaufmann et al., "Specific Proteolytic Cleavage of Poly (ADP-ribose) Polymerase: An Early Marker of Chemotherapy-induced Apoptosis", Cancer Res., 1993, 53, 3976-3985.
Lamarre et al., "Production and characterization of monoclonal antibodies specific for the functional domains of poly (ADP-ribose) polymerase", Biochem. Cell Biol., 1986, 64, 368-376.
Lassmann et al., "Cell death in Alzheimer's disease evaluated by DNA fragmentation in situ", Acta. Neuropathol., 1995, 89, 35-41.
Lazebnik et al., "Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE", Nature, 1994, 371, 346-347.
Lippke et al., "Identification and Characterization of CPP32/Mch2 Homolog 1, a Novel Cysteine Protease Similar to CPP32", J. Biol. Chem., 1996, 271, (4) , 1825-1828.
Los et al., "Requirement of an ICE/CED-3 protease for Fas/APO-1-medicated apoptosis", Nature, 1995, 375, 81-83.
Malik et al., "Antibody to poly(adenosine diphosphate-ribose) polymerase and its use in chromatin analysis", Nucl. Acid Res., 1982, 10(9), 2939-2950.
Martin et al., "Protease Activation during Apoptosis: Death by a Thousand Cuts?", Cell, 1995, 82, 349-352.
Meyer et al., "Enzymatic Properties of Recombiant ICH-1L Support a Role in Mediating Apoptosis", Soc. Neurosci., 1996, 22, 565, Abstract No. 228.3.
Milligan et al., "Peptide Inhibitors of the ICE Protease Family Arrest Programmed Cell Death of Motoneurons In Vivo and Vitro", Neuron, 1995, 15, 385-393.
Miura et al., "Introduction of Apoptosis in Fibroblasts by IL-1.beta.-Converting Enzyme, a Mammalian Homolog of the C. elegans Cell Death Gene ced-3", Cell, 1993, 75, 653-660.
Muzio et al., "Flice, A Novel FADD-Homologous ICE/CED-3-like Protease, is Recruited to the CD95 (Fas/APO-1) Death-Inducing Signaling Complex", Cell, 1996, 85, 817-827.
Nicholson et al., "Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis", Nature, 1995, 376, 37-43.
Nicoletti et al., "A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry", J. Immunol. Methods, 1991, 139, 271-279.
Nitatori et al., "Delayed Neuronal Death in the CA1 Pyramidal Cell Layer of the Gerbil Hippocampus following Transient Ischemia is Apoptosis", J. Neurosci., 1995, 15(2) , 1001-1011.
Oppenheim, "Cell Death During Development of the Nervous System", Annu. Rev. Neurosci., 1991, 14, 453-501.
Roberts-Lewis et al., "Immunolocalization of Calpain I-mediated Spectrin Degradation to Vulnerable Neurons in the Ischemic Gerbil Brain", J. Neurosci., 1994, 14(6) , 3934-3944.
Saito et al., "Cloning of a full-length cDNA encoding bovine thymus poly(ADP-ribose) synthetase: evolutionarily conserved segments and their potential functions", Gene, 1990, 90, 249-254.
Schlegel et al., "CPP32/Apopain Is a Key Interleukin 1.beta. Converting Enzyme-like Protease Involved in Fas-mediated Apoptosis", J. Biol. Chem., 1996, 271(4), 1841-1844.
Schulze-Osthoff et al., "Cell Nucleus and DNA Fragmentation Are Not Required for Apoptosis", J. Cell Biol., 1994, 127(1), 15-20.
Shah et al., "Detection of Poly(ADP-Ribose) Polymerase and Its Apoptosis-Specific Fragment by a Nonisotopic Activity-Western Blot Technique", Analyt. Biochem., 1995, 232, 251-254.
Tewari et al., "Yama/CPP32.beta., a Mammalian Homolog of CED-3, Is a CrmA-Inhibitable Protease That Cleaves the Death Substrate Poly (ADP-Ribose) Polymerase", Cell, 1995, 81, 801-809.
Thompson, "Apoptosis in the Pathogenesis and Treatment of Disease", Science, 1995, 267, 1456-1462.
Troy et al., "The contrasting roles of ICE family proteases and interleukin-1.beta. in apoptosis induced by trophic factor withdrawal and copper/zinc superoxide dismutase down-regulation", Proc. Natl. Acad. Sci. USA, 1996, 93, 5635-5640.
Wang et al., "Ich-1, an ICE/ced-3-Related Gene, Encodes Both Positive and Negative Regulators of Programmed Cell Death", Cell, 1994, 78, 739-750.
Wijsman et al., "A New Method to Detect Apoptosis in Paraffin Sections: In Situ End-labeling of Fragmented DNA", J. Histochem. Cytochem., 1993, 41(1), 7-12.
Woo, "Apoptosis and Loss of Renal Tissue in Polycystic Kidney Disea

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