Methods for detecting bioactive compounds

Details Methods for detecting bioactive compounds Methods for detecting bioactive compounds

2005-07-05

2005-07-05

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

C435S040500, C435S029000, C424S489000, C424S520000

Reexamination Certificate

active

06913877

ABSTRACT:
Methods of detecting bioactive compounds include exposing compounds to one or more classes of chromatophores and measuring or sensing associated changes in one or more of the chromatophores. Representative methods permit identification and quantification of neurotransmitters, toxins, hormones, and chemical warfare agents with or without prior knowledge of the content of a sample. In some examples, chromatophores based on Betta fish are used. Cytosensor apparatus using such chromatophores include means for exposing chromatophores to a sample and optical detection systems for assessing changes in chromatophore optical properties. Compounds can be identified or quantified based on a red-green-blue or hue saturation-value representations of transmitted or reflected light or based on other characterizations of transmitted or reflected light.

REFERENCES:
patent: 4401755 (1983-08-01), Weaver
patent: 4985353 (1991-01-01), Elving
patent: 5462856 (1995-10-01), Lerner et al.
patent: 5641644 (1997-06-01), Klebe
patent: 5721135 (1998-02-01), Thastrup et al.
patent: 5900361 (1999-05-01), Klebe
patent: 5919646 (1999-07-01), Okun et al.
patent: 5998212 (1999-12-01), Corio et al.
Paul, Vignais; “Photo phosphorylation in bacterial chromatophores entrapped in alginate gel improvement of the physical and biochemical properties of gel beads with barium as gel inducing agent”, Enzyme and Microbial Technology, 1980, vol. 2, No. 4, p. 281-87.
Kotz et al., “Intracellular calcium and cAMP regulated directional pigmetn movements in teleost erythrophores”, J Cell Biology, 1994, vol. 124, No. 4, pp. 463-474.
Karlsson et al., “The melanophore aggregating response of isolated fish scares: a very rapid and sensitive diagnosis of whooping cough,”FEMS Microbiology Letters82:169-176 (1991).
Karlsson et al., “The Effect of Pertussis Toxin on Alpha-2-Adrenoceptor-Mediated Pigment Migration in Fish Mediated Pigment Migration in Fish Melanophores,”Life Science37:1043-1949 (1985).
Karlsson et al., “Pronounced Supersensitivity of Postjunctional Alpha Adrenoceptors after Denervation of Fish Melanophores,”The Journal of Pharmacology and Experimental Therapeutics246:345-351 (1988).
Land, M.F., “The Physics and Biology of Animal Reflectors,”0Dept. of Physiology, University of California, Berkeley,pp. 75-105.
Lu et al., “Single-Molecule Enzymatic Dynamics,”Science282:1877-1881 (Dec. 1998).
Maeno et al., “Adrenergic Mechanisms Associated with the Movement of Platelets and Iridophores from the Freshwater Goby,Odontobutis Obscura,” Comp. Biochem. Physiol.102C:233-237 (1992).
Martensson, et al., “Denervation of Pigment Cells Lead to a Receptor that is Ultrasensitive to Melatonin and Noradrenaline,”Life Sciences18:1575-1582 (1997).
Oshima and Nagaisha, “Study of the Motile Mechanism in Neon Tetra (Paracheirodon innesi) Iridophores,”Comp. Biochem. Physiol.102A:273-278 (1992).
Potenza et al., “A Method for Evaluating the Effects of Ligands upon GSProtein-Coupled Receptors Using a Recombinant Melanophre-Based Bioassy,”Anal. Biochem.206:315-322 (1992).
“Axiom Biotechnologies Issued U.S. Patent on High Throughput Pharmacy System (HT-PS™)” Axiom Biotechnologies, Inc., Apr. 3, 2000, http://www.axiombio.com/home.html.
Clothier et al., “Light-induced colour changes by the iridophores of the Neon tetra,Paracheirodon innesi,” J. Cell Sci.88:663-668 (1987).
Danosky et al., “Biosensors based on the chromatic activities of living, naturally pigmented cells: digital image processing of the dynamics of fish melanophores,”Biosensors&Bioelectronics12:925-936 (1997).
Elwing et al., “Fish Scales as Biosensors for Catecholamines,”Biosensors&Bioelectronics5:449-459 (1990).
Fujii, R., “Coloration and Chromatophores” inThe Physiology of Fishes,D.H. Evans, ed., (CRC Press, 1993).
Fujii et al, “Control of Chromatophore Movements in Telecost Fishes,”Zoological Science3:13-47 (1986).
Fujii et al., “Cytophysiology of Fish Chromatophores,”International Review of Cytology143:191-255 (1993).
Jayawickreme et al., “Creation and functional screening of a multi-use peptide library,”Proc. Natl. Acad. Sci. USA91:1614-1618 (1994).
Jayawickreme et al. “Discovery and Structure-Function Analysis of α-Melanocyte-stimulating Hormone Antagonists,”J. Biological Chemistry269:29846-29854 (1994).
Quillan et al., “Combinatorial diffusion assay used to identify topically active melanocyte-stimulating hormone receptor antagonists,”Proc. Natl. Acad. Sci USA92:2894-2898 (1995).
Rohrlich, S.T., “Fine Structural Demonstration of Ordered Arrays of Cytoplasmic Filaments in Vertebrate Iridophores,”J. Cell Bio.62:295-304 (1974).
Svensson et al., “Melanophores in Isolated Scales ofLabrus Berggylta(Ascanius): Innervation and Alpha2-Adrenoceptor-Mediated Pigment Aggregation,”Comp. Biochem. Physiol.930:247-251 (1988).
Yamada et al., “Autoradiographic Demonstration of Adrenergic Innervation to Scale Melanophores of a Teleost Fish,Oryzias latipes,” J. Exp. Zoology229:73-80 (1984).
“The New English Bible”, Oxford University, Genesis, pp. 33-34, (1970). (See especially p. 33, line 46 through p. 34, line 25).

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