Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2004-07-02
2010-11-16
Myers, Carla (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C436S501000, C436S164000, C436S172000
Reexamination Certificate
active
07833704
ABSTRACT:
The present invention provides a method for the detection and sorting of microparticles in a mixture of microparticles. The method of the present invention allows for the detection and sorting of many distinct microparticle classes. Detection and sorting is on the basis of microparticle size, the fluorescence spectrum of any attached reporter molecule, the fluorescence intensity of the reporter molecule, and the number of particles in each classification bin. These microparticle classes have particular applications in many genetic or biochemical multiplexing studies and especially as binding agents for the detection of aneuploidy in an organism or embryo of the organism. In humans, the detection and sorting of at least 24 classes of microparticles would be sufficient for a single tube method for the simultaneous detection of aneuploidy in all chromosomes, wherein each distinct microparticle class comprises a polynucleotide sequence complementary to, and specific for, a polynucleotide sequence that is unique to a particular human chromosome. Furthermore, using currently available technology, the present method has application for the simultaneous detection of aneuploidy in all chromosomes for an organism that has 216 or fewer pairs of chromosomes. Kits for the simultaneous detection of aneuploidy in one or more human chromosomes are also provided.
REFERENCES:
patent: 5756696 (1998-05-01), Gray et al.
patent: 6562565 (2003-05-01), Pinkel et al.
patent: 6787307 (2004-09-01), Bitner et al.
patent: 2003/0124584 (2003-07-01), Mohammed
patent: 2007/0015159 (2007-01-01), Wildenberg et al.
patent: 2008/0102455 (2008-05-01), Poetter
patent: WO 95/08646 (1995-03-01), None
patent: WO 97/14028 (1997-04-01), None
patent: WO 01/27328 (2001-04-01), None
patent: WO 02/40698 (2002-05-01), None
patent: WO 02/068685 (2002-09-01), None
Ibanez E, et al. Assessment of the proportion of transgene-bearing sperm by fluorescence in situ hybridization: a novel approach for the detection of germline mosaicism in transgenic male founders. Mol Reprod Dev. Feb. 2001;58(2):166-72.
Gvakharia M, et. al. Single in vitro fertilization (IVF) cycle with blastomere biopsy for preimplantation genetic diagnosis (PGD) of Huntington's disease, assisted hatching and cryopreservation results in healthy baby and subsequent ongoing pregnancy. Fertility and Sterility. Sep. 2002; 78(Supplement 1):S229.
Bianchi et al.,Prenat. Diag.22(7):609-615 (2002).
Bonner and Laskey,Eur. J. Biochem46:83-88 (1974).
Cook, E.B. et al., Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs. non-allergics.Journal of Immunological Methods,254:109-118 (2001).
Gray, J.W. et al.,Clinical Chemistry,21(9): 1258-1262 (1975).
He, H. et al.,International Journal of Radiation Biology,77(7):787-795 (2001).
Kraus, M. et al.,E Experimental Technique of Physics/Experimentelle Technik Der Physik,41(1):139-153 (1995).
Lomax, B. et al., Comparative genomic hybridization in combination with flow cytometry improves results of cytogenetic analysis of spontaneous abortions.American Journal of Human Genetics66:1516-1521 (2000).
Marmur and Doty, J. Mol. Biol. 5:109-118 (1962).
Schmidt, W. et al.,Mol. Hum. Reprod.6(9):855-860 (2000).
Spiro, A. et al., A bead-based method for multiplexed identification and quantification of DNA sequences using flow cytometry,Applied and Environmental Microbiology66(10):4258-4265 (2000).
Stepanov, S.I. et al.,Cytometry,23(4): 279-283 (1996).
Trask, B. et al.,Cytometry11:184-195. (1990).
Voojis, M. et al., AM. J. Hum. Genet. 52: 586-597 (1993).
Wilton, L., Preimplantation genetic diagnosis for aneuploidy screening in early human embryos: a review,Prenatal Diagnosis22:512-518 (2002).
Spiro, et al, A Bead-Based Method for Multiplexed Identification and Quantitation of DNA Sequences Using Flow Cytometry, Applied and Environmental Biology, Oct. 2000, p. 4258-4265, vol. 66, No. 10.
Poetter Karl
Wildenberg Andrew Patrick
Genera Biosystems Limited
McCallum Esq. Jennifer M.
Myers Carla
The McCallum Law Firm, P.C.
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