Methods for detecting and isolating antigen-specific T...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007100, C435S007200, C435S007210, C435S375000, C435S377000

Reexamination Certificate

active

07659084

ABSTRACT:
What is suggested herein is the use of CD154 for the detection and/or isolation of antigen-specific T lymphocytes and a method for the detection and/or isolation of antigen-specific T lymphocytes, wherein the suspension is contacted with a CD40/CD154 system inhibitor, intra- or extracellular determination of CD154 is effected, and the cells having CD154 are detected and/or isolated.

REFERENCES:
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patent: 2003/0012781 (2003-01-01), Darrell et al.
patent: WO 9958977 (1999-11-01), None
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XP000887155— Valmori et al.; “An Antigen-targeted Approach to Adoptive Transfer Therapy of Cancer”; Cancer Research, vol. 59, May 1999; pp. 2167-2173.
XP002220000; Lee et al.; “The biological outcome of CD40 signaling in B lymphocytes is dependent on the duration of CD154 expression on T cells: Reciprocal regulation by 1L-4 and IL-12”; FASEB Journal; Mar., 2002; 1 p.
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Database Biosis ′Online! Biosciences Inforamtion Service, Philadelphia, PA, US; Mar. 20, 2002, Lee Byung O et al.: “The Biological Outcome of CD40 Signaling in B Lymphocytes is Dependent on the Duration of CD154 Expression on T Cells: Reciprocal Regulation by IL-4 and IL-12.”
FASEB Journal, vol. 16, No. 4, Mar. 20, 2002, p. A350, Annual Meeting of the Professional Research Scientists on Experimental Biology; New Orleans, Louisiana, USA; Apr. 20-24, Mar. 20, 2002.
Valmori D et al: “Antigen-Targeted Approach to Adoptive Transfer Therapy of Cancer” Cancer Research, American Association for Cancer Research, Baltimore, MD, vol. 59, No. 9, 1999, pp. 2167-2173.

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