Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Reexamination Certificate
2004-10-13
2009-08-25
Tsang, Cecilia (Department: 1654)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
C435S007500, C435S018000, C435S023000, C435S024000, C530S300000, C530S324000, C530S325000, C530S326000, C530S327000, C530S328000, C530S329000, C530S406000
Reexamination Certificate
active
07579165
ABSTRACT:
The present invention provides a method for effectively introducing sulfonic acid groups into the N-terminus of a protein or a peptide. The method comprises a modification step to react a N-terminus in a protein or peptide with a compound A which includes disulfide group and a cleavage step to cleave a disulfide bond of the disulfide group to convert into a sulfonic acid group. The present invention also provides a method of analyzing proteins or peptides easily and effectively on mass spectrometry and an intermediate that can be used to effectively derivatize proteins or peptides as sulfonic acid derivatives.
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T. Keough et al., “A Method for High-Sensitivity Peptide Sequencing Using Postsource Decay Matrix-Assisted Laser Desorption Ionization Mass Spectrometry”, Proceeding of the National Academy of Science of the United States of America, vol. 96, pp. 7131-7136, Jun. 1999.
Ando Eiji
Kuyama Hiroki
Nakazawa Takashi
Norioka Shigemi
Obama Takashi
Cheng Law Group PLLC
Ha Julie
Shimadzu Corporation
Tsang Cecilia
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