Methods for delivering biologically active molecules into cells

Details Methods for delivering biologically active molecules into cells Methods for delivering biologically active molecules into cells

2005-12-13

2005-12-13

Ketter, James (Department: 1633)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S455000, C536S023100

Reexamination Certificate

active

06974698

ABSTRACT:
Methods for delivering a biologically active molecule into a cell by linking a molecule to the cell surface, wherein the molecule can act as a surface receptor, then complexing the biologically active molecule with a ligand for the surface receptor, and finally contacting the biologically active molecule-ligand complex with the cell surface are disclosed. Delivery of any biologically active molecule, e.g. proteins, enzymes, nucleic acids, hormones, nucleic acids, and oligonucleotides, is contemplated. The use of biotin or biotinylated antibodies as the surface receptor is disclosed. The use of PEI and PEI-avidin conjugates complexed with oligonucleotides for delivery into a directly or indirectly biotinylated cell surface, along with the PEI-avidin-nucleic acid compositions, are disclosed. Primary and cultured cells with a covalently linked surface receptor molecule, such as biotin, on their surfaces are also disclosed.

REFERENCES:
patent: 4996142 (1991-02-01), Al-Hakim et al.
patent: 5108921 (1992-04-01), Low et al.
patent: 5254342 (1993-10-01), Shen et al.
patent: 5766902 (1998-06-01), Craig et al.
patent: 6045795 (2000-04-01), Ulevitch et al.
Karlsson, et al. APMIS. 1992, Suppl.27, vol. 100, pp. 71-83.
Keusch, Gerald. Reviews of Infectious Diseases. 1979, vol. 1, No. 3, pp. 517-529.
Hermanson, G.T. Bioconjugate Techniques. 1996, Academic Press, Inc. pp. 3-136, 371-401, 570-571, 575-584 and 590-592.
Palu, et al. Journal of Biotechnology, 1999, vol. 68, pp. 1-13.
Luo, et al. Nature Biotechnology, Jan. 2000, vol. 18, pp. 33-37.
Verma, et al. Nature, 1997, vol. 389, pp. 239-242.
Wojda, et al. Molecular Biology of the Cell, Nov. 1997, vol. 8, No. Suppl., p86A.
Dachs et al. Oncology Research. 1997, vol. 9, pp. 313-325.
Schwartzenberger, et al. Journal of Virology. 1997, vol. 71, No. 11, pp. 8563-8571.
Spear, et al. Heparin and Related Polysaccharides. D.A. Lane editor, Plennum Press, 1992, pp. 341-353.
Baker, A., M. Saltik, H. Lehrmann, I. Killisch, V. Mautner, G. Lamm, G. Christofori, M. Cotten, “Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery.”Gene Therapy4:773-782 (1997).
Ferro, W.G., S. Li, J.D. Rosenblatt, N. Sirianni, J.E. Morgan, T.A. Partridge, L. Huang, E.P. Hoffman, “Selection and use of ligands for receptor-mediated gene delivery to myogenic cells.”Gene Therapy4:664-674 (1997).
Franco, Robert S., J. Lohmann, E. B. Silberstein, G. Mayfield-Pratt, M. Palascak, T. A. Nemeth, C. H. Joiner, M. Weiner, D. L. Rucknagel, “Time-dependent Changes in the Density and Hemoglobin F. Content of Biotin-Labeled Sickle Cells.”The Journal of Clinical Investigation101 No. 12:2730-2740 (1998).
Kircheis, R., Kichler, A., Wallner, G. M. Kursa, M. Ogris, T. Felzmann, M. Buchberger and E. Wagner, “Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery.”Gene Therapy4:409-418 (1997).
Kukowska-Latallo, J.F., A. U. Bielinska, J. Johnson, R. Spindler, D. A. Tomalia, J. R. Baker, Jr., “Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers.”Genetics93:4897-4902 (May 1996).
Schoeman, R., D. Joubert, M. Ariatti, A. O. Hawtrey, “Further Studies on Targeted DNA Transfer to Cells Using a Highly Efficient Delivery System of Biotinylated Transferrin and Biotinylated Polylysine Complexed to Streptavidin.”Journal of Drug Targeting4:509-516 1995).
Youssoufian, H., F. A.E. Kruyt, Xiaotong Li, “Protein Replacement by Receptor-Mediated Endocytosis Corrects the Sensitivity of Fanconi Anemia Group C Cells to Mitomycin C.”Blood93:363-369 No. 1 (Jan. 1, 1999).
Zanta, M.A., O. Boussif, A. Adib, J.P. Behr, “In Vitro Gene Delivery to Hepatocytes with Galactosylated Polyethylenimine.”Bioconjugate Chem.8:839-844 (1997).

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