Methods for culturing hair follicle epithelial matrix cells

Details Methods for culturing hair follicle epithelial matrix cells Methods for culturing hair follicle epithelial matrix cells

1993-12-03

1996-11-05

Jordan, Charles T.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524021, 43524023, 435240243, 4352403, 43524031, C12N 502

Patent

active

H00016101

ABSTRACT:
The subject invention relates to methods of isolating, culturing and propagating epithelial matrix cells by removing/isolating the bulb region of a hair follicle directly above the dermal papilla, incubating the bulb in a pre-coated culture dish containing Chang medium supplemented with 10% fetal bovine serum, replacing the Chang medium with keratinocyte growth medium after 3-4 days, propagating the cells to the next passage after adding the keratinocyte growth medium and passing the pure epithelial matrix cells again to obtain a third passage of cells. The epithelial matrix cells may be passed for up to six passages, and are useful for screening potential hair growth actives.

REFERENCES:
patent: 4254226 (1981-03-01), Eisinger et al.
patent: 4919664 (1990-04-01), Oliver et al.
patent: 5130142 (1992-07-01), Wong et al.
patent: 5229271 (1993-07-01), Philpott
Yuspa et al, NTIS patent application 07/048,537, filed May 6, 1987.
Warren et al., J. Invest. Dermatology, vol. 98, No. 5, pp. 693-699 (May 1992).
Parkinson, Seminars in Cell Biology, vol. 3 pp. 435-444 (1992).
Chang et al. PNAS (USA), vol. 79, pp. 4795-4799 (Aug. 1982).
Detmar et al, J. Invest. Dermatol. vol. 101; pp. 130S-134S (1993).
Frater, R. "In Vitro Differentiation of Mouse Hair Follicle Cells", J. of Investigative Derm., vol.64, No. 4, pp. 235-239 (1975).
Frater, R. "A New Technique for Dissociation of Hair Follicles into Single Cells", Experientia, vol. 32, pp. 675-676 (1976).
Weterings, P. J. J. M., A. J. M. Vermorken and H. Bloemendal, "A Method for Culturing Human Hair Follicle Cells", British J. of Derm., vol. 104, pp. 1-5 (1981).
Hewish D. R. and R. C. Marshall, "In Vitro Growth and Differentiation of Epithelial Cells Derived From Post-Embryonic Hair Follicles", Aust. J. Biol. Sci., vol. 35, pp. 103-109 (1982).
Jones, L. N., K. J. Fowler, R. C. Marshall and M. L. Ackland, "Studies of Developing Human Hair Shaft Cells In Vitro", The Society of Investigative Dermatology, vol. 1, pp. 58-64 (1988).
Schaart, F. M., A. Mayer-da-Silva and C. E. Orfanos "Cultivation of Human Hair Follicle Cells", Hair and Hair Diseases, C. E. Orfanos and R. Happle, (eds) Chap. 13, pp. 301-324, (1989).
Philpott M. P., M. R. Green and T. Kealey "Human Hair Growth In Vitro", J. of Cell Sci., vol. 97, pp. 463-471 (1990).
Kurata, S., S. Itami, T. Sonoda and S. Takayasu "Culture and Differentiation of Human Hair Matrix Cells In Vitro", Abstracts, World Congress of Dermatology, Jun. 12-18, NY (1992).
Reynolds, A. J., C. M. Lawrence and A. B. Jahoda "Human Hair Follicle Germinative Epidermal Cell Culture", J. of Invest. Derm., vol. 101, No.4, pp.634-638 (1993).

Affiliated with

Also associated with

Rating

Methods for culturing hair follicle epithelial matrix cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for culturing hair follicle epithelial matrix cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for culturing hair follicle epithelial matrix cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2006146