Methods for creating transgenic animals

Details Methods for creating transgenic animals Methods for creating transgenic animals

1997-03-20

2000-06-27

Campbell, Bruce R.

Multicellular living organisms and unmodified parts thereof and

Method of making a transgenic nonhuman animal

Via retrovirus

435456, C12N 1500

Patent

active

060809124

ABSTRACT:
The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.

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