Methods for controlling sialic acid derivatives in recombinant g

Chemistry: molecular biology and microbiology – Condition responsive control process

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435 4, 435 721, 435 694, 435 701, 435 703, 4352402, C12Q 300, C12Q 100, C12N 500, C12P 2100

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054590310

ABSTRACT:
The invention relates to the effects of certain cell culture and/or production parameters on the carbohydrate composition of a cell culture-derived recombinant glycoprotein. The methods of the invention demonstrate a practical method for controlling levels of a sialic acid derivative, NGNA, in secreted recombinant glycoproteins which will be useful in a variety of bioprocessing systems.

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Derwent Abs. 87-353301/50 Otsuka Pharm KK (J62258324) Nov. 10, 1987.
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Hokke et al, FEBS, 275:9-14 (1990).
Tsao et al., Annals N.Y. Academy Science, 665:127-136 (1992).
Goochee, C. and Monica, T., Biotechnology, 8:421-427 (1990).
Derwent Abstract 87-151423 Otsuka Pharm KK J62258324 (Nov. 10, 1987).

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