Methods for constructing DNA library and support carrying...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C435S005000, C435S004000, C435S091200, C435S091510, C435S091500, C435S091500, C536S023100, C536S024100, C536S024200

Reexamination Certificate

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07029881

ABSTRACT:
Method and apparatus for constructing a cDNA library by hybridizing mRNA with oligo (dT) on a support and treating with a reverse transcriptase to immobilized complementary DNA, or for constructing a gDNA library by ligating a double-stranded chromosomal DNA library with an oligonucleotide on a support having a restriction enzyme site and then immobilizing the gDNA library.

REFERENCES:
patent: 5656462 (1997-08-01), Keller et al.
patent: 6225077 (2001-05-01), Schmidt et al.
patent: 6489111 (2002-12-01), Takahashi et al.
patent: 0 701 001 (1996-03-01), None
patent: WO 99/63072 (1999-12-01), None
patent: WO 99/41362 (2000-11-01), None
Schena, M. et al. “Parallel Human Genome Analysis: Microarray-beased expression Monitoring of 1,000 Genes,” Proc. Natl. Acad. Sci. USA (1996) vol. 93, P. 10614-10619 Schena, M. et al.

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