Methods for conducting specific binding assays

Details Methods for conducting specific binding assays Methods for conducting specific binding assays

1987-02-20

1989-09-19

Kepplinger, Esther M.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

436518, 436536, 436538, 436806, 436824, 435 7, G01N 33546

Patent

active

048681300

ABSTRACT:
Methods and devices for separating bound label from unbound label within an assay mixture and for predispensing assay reactants in self-contained assay vessels, as well as a method for detecting the presence and/or amount of an analyte within a fluid sample, and a reusable detection vessel for use therein and with specific binding assays in general are disclosed. Significant to the separation of bound label from unbound label is the use of a cushion comprising generally one primary layer and in some cases one or more secondary layers.

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R. S. Yalow, "Radioimmunoassay: A Probe for the Fine Structure of Biologic Systems", Science, 200: 1236-1245, 1978.

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