Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-02-27
2000-02-22
Arthur, Lisa B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 9131, 435 911, 435 9151, 514 44, C12Q 168, C12P 1934, A01N 4304
Patent
active
060278951
ABSTRACT:
The present invention provides new methods, employing a nucleotide integrase, for cleaving single-stranded RNA substrates, single-stranded DNA substrates, and double- stranded DNA substrates at specific sites and for inserting a nucleic acid molecule into the cleaved substrate. One method uses a nucleotide integrase to cleave one strand of a double-stranded DNA substrate. The method comprises the steps of: providing a nucleotide integrase comprising a group II intron RNA having two hybridizing sequences that are capable of hybridizing with two intron RNA binding sequences on the one strand of the DNA substrate, and a group II-intron encoded protein which binds to a first sequence element of the substrate; and reacting the nucleotide integrase with the double-stranded DNA substrate under conditions that permit the nucleotide integrase to cleave the one strand of the DNA substrate and to insert the group II intron RNA into the cleavage site. The method of cleaving both strands of a double-stranded DNA substrate comprises the steps of: providing a nucleotide integrase comprising a group II intron RNA having two hybridizing sequences that are capable of hybridizing with two intron RNA binding sequences on one strand of the substrate, and a group II-intron encoded protein that is capable of binding to a first sequence element and to a second sequence element in the recognition site of the substrate; and reacting the nucleotide integrase with the double-stranded DNA substrate such that the nucleotide integrase cleaves both strands of the DNA substrate and inserts the group II intron RNA into the cleavage site of the one strand. The method for cleaving a single-stranded nucleic acid substrate comprises the steps of: providing a nucleotide integrase having two hybridizing sequences that are capable of hybridizing with two intron RNA-binding sequences on the single-stranded substrate, and a group II intron encoded protein; and reacting the nucleotide integrase with the single stranded nucleic acid substrate for a time and at a temperature sufficient to allow the nucleotide integrase to cleave the substrate and to attach the group II intron RNA molecule thereto.
REFERENCES:
patent: 5498531 (1996-03-01), Jarrell
patent: 5698421 (1997-12-01), Lambowitz et al.
Lazowska et al.; The EMBO Journal, vol. 13, pp 4963-4972, 1994.
"A bacterial group II intron encoding reverse transcriptase, maturase, and DNA endonuclease activities: biochemical demonstration of maturase activity and insertion of new genetic information within the intron" by Matsuura, et al., Genes & Development, vol. 11, 1997, pp. 2910-2924.
"Group II intron endonucleases use both RNA and protein subunits for recognition of specific sequences in double-stranded DNA" by Guo, et al., The EMBO Journal, vol. 16, No. 22, 1997, pp. 6835-6848.
"Efficient integration of an intron RNA into double-stranded DNA by reverse splicing" by Yang, et al., Nature, vol. 381, No. 23, May 1996, pp. 332-335.
"Mobility of Yeast Mitochondrial Group II Introns: Engineering a New Site Specifically and Retrohoming via Full Reverse Splicing" by Eskes, et al., Cell, vol. 88, Mar. 21, 1997, pp. 865-874.
"Splicing of a Group II Intron Involved in the Conjugative Transfer of pRS01 in Lactococci" by Mills, et al., Journal of Bacteriology, vol. 178, No. 12, Jun. 1996, pp. 3531-3538.
"Splicing of a group II intron in a functional transfer gene of Lactococcus lactis" by Shearman, et al., Molecular Microbiology, vol. 21, 1996, pp. 45-53.
"Targeted DNA cleavage and insertion by group II introns" by Guo, et al., RNA '97, The second annual meeting of the RNA society, May 27-Jun. 1, 1997, p. 262.
"A bacterial group II intorn encoding reverse transcriptase, maturase, and DNA endonuclease activities" by Matsuura, et al., RNA '97, The second annual meeting of the RNA society, May 27-Jun. 1, 1997, p. 369.
"Novel mechanisms for site-specific DNA cleavage and insertion involving group II intron catalytic RNAs" by Lambowitz, Functional Genomics Conference, Newport, Rhode Island, Nov. 2-5, 1997.
"Group II introns as site-specific retroelements", by Guo, et al., Keystone Symposia on Molecular and Cellular Biology: Transposition and Site-Specific Recombination, Santa Fe, New Mexico, Mar. 1-7, 1997.
"Group II intorn mobility and evolution" by Zimmerly, et al., Keystone Symposia on Molecular and Cellular Biology: Posttranscriptional RNA Processing, Hilton Head, South Carolina, Mach 11-17, 1996.
"Retrotransposition of group II introns" by Zimmerly, et al., Keystone Symposia on Molecular and Cellular Biology: Viral Genome Replication, Tamarron, Colorado, Mar. 1-7, 1996.
"Mitochondrial plasmid and group II intron reverse transcription" by Lambowitz, et al., Keystone Symposia on Molecular and Cellular Biology: Viral Genome Replication, Tamorron, Colorado, Mar. 1-7, 1996.
"Homing of a Group II Intron from Lactococcus lactis subsp. lactis ML3" by Mills, et al., Journal of Bacteriology, Oct. 1997, vol. 179, No. 19, pp. 6107-6111.
Executed Supplemental Information Disclosure Statement of Steven Zimmerly.
Executed Supplemental Information Disclosure Statement of Alan Lambowitz.
"Group I and group II introns" by Saldanha, et al., The FASEB Journal, vol. 7, Jan., 1993, pp. 15-24.
"Group II Intron Mobility Occurs to Target DNA-Primed Reverse Transcription" by Zimmerly, et al., Cell, vol. 82, Aug. 25, 1995, pp. 545-554.
"An Expanding Universe of Introns" by Belfort, Science, vol. 262, Nov. 12, 1993, pp. 1009-1010.
"Integration of Group II Intron b11 into a Foreign RNA by Reversal of the Self-Splicing Reaction in Vitro", by Morl and Schmelzer, Cell, vol. 60, Feb. 23, 1990, pp. 629-636.
"A Group II Intron RNA is a Catalytic Component of a DNA Endonuclease Involved in Intron Mobility", by Zimmerly, et al., Cell, vol. 83, Nov. 17, 1995, pp. 1-10.
"RNA enzymes (ribozymes) as antiviral therapeutic agents" by Rossi and Sarver, Tibtech, vol. 8, Jul., 1990, pp. 179-183.
"Mobile Group II Introns of Yeast Mitochondrial DNA Are Novel Site-Specific Retroelements" by Moran, et al., Molecular and Cellular Biology, vol. 15, No. 5, May 1995, pp. 2828-2838.
"Evolutionary relationships among group II intorn-encoded proteins and identification of a conserved domain that may be related to maturase function" by Mohr, et al., Nucleic Acids Research, vol. 21, No. 22, 1993, pp. 4991-4997.
"Reverse Trnascriptase Activity Associated with Maturase-Encoding Group II Introns in Yeast Nitochondria" by Kennell, et al., Cell, vol. 73, Apr. 9, 1993, pp. 133-146.
"Introns as Mobile Genetic Elements" by Lambowitz, et al., Annual Reviews Biochem., vol. 62, 1993, pp. 587-622.
Beall Clifford James
Gau Huatao
Lambowitz Allen M.
Mohr Georg
Zimmerly Steven
Arthur Lisa B.
Souaya Jehanne
The Ohio State University Research Foundation
LandOfFree
Methods for cleaving DNA with nucleotide integrases does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for cleaving DNA with nucleotide integrases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for cleaving DNA with nucleotide integrases will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-519504