Methods for characterizing phosphorothioate oligonucleotides

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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536 253, 536 254, 435 911, C07H 2100, C07H 2104

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056291507

ABSTRACT:
This invention presents novel methods for desulfurizing and sequencing phosphorothioate oligonucleotides. These methods allow easy access to the analysis of phosphorothioate oligonucleotides because these methods may be performed as a final step in oligonucleotide synthesis. The methods of the invention will permit rapid characterization of oligonucleotides and may be easily adapted to characterizing pharmaceutical formulations.

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