Methods for characterizing glycosylation sites

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007100, C436S501000, C436S518000, C422S050000, C422S067000, C424S009100

Reexamination Certificate

active

07820399

ABSTRACT:
The present invention provides methods for isolating and characterizing the glycosylation sites of a glycoprotein, such as a glycosylated antibody. In particular, the methods employ affinity capture, liquid chromatography, and mass spectrometry to determine, for example, the location of the glycopeptide, the heterogeneity of the glycan attached to the glycopeptide, the mass of the glycopeptide, and/or the peptide sequence.

REFERENCES:
patent: 07083922 (1995-03-01), None
Mathov et al. (Molecular Immunology, vol. 32, No. 14/15, pp. 1123-1130, 1995).

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