Methods for capture and selective release of nucleic acids using

Details Methods for capture and selective release of nucleic acids using Methods for capture and selective release of nucleic acids using

1994-09-15

1996-12-10

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, C12Q 168, C12Q 170

Patent

active

055829880

ABSTRACT:
Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with specific weakly basic polymers to form a precipitate with the nucleic acids at acidic pH. After removing non-precipitated materials, the pH is then made basic, thereby releasing the nucleic acids from the polymer. This method for preparing specimen samples is simple and quite rapid, and the released nucleic acids can be further treated in hybridization assays or amplification procedures. The weakly basic polymers are water-soluble and cationic at acidic pH, but neutral in charge at basic pH.

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patent: 4839231 (1989-06-01), Vandekerckhove
patent: 4994192 (1991-02-01), Corin et al.
patent: 5053326 (1991-10-01), Renz

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