Methods for capture and selective release of nucleic acids using

Details Methods for capture and selective release of nucleic acids using Methods for capture and selective release of nucleic acids using

1994-09-13

1997-04-22

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 912, 435 911, 536 243, 536 2432, 536 2433, 424 941, C12Q 168, C12Q 170, C12P 1934, C07H 2104

Patent

active

056228228

ABSTRACT:
Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with polyethyleneimine to form a precipitate with the nucleic acids. The nucleic acids are then released from the precipitate by contact with a strong base, and the released nucleic acids are kept in solution with an anionic phosphate ester surfactant. This method for preparing specimen samples is simple and quite rapid.

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Hoopes and McClure, NAR 9: 5493-5504. 1981.
Scopes, In PRotein Purification: PRinciples and Practice, Springer Verlag, pp. 100-114. 1987.
Renz et al, Nucl Acids Res., 12, pp. 3435-3444, 1984.

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