Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including freezing; composition therefor
Patent
1996-01-11
1999-01-26
Saucier, Sandra E.
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
Including freezing; composition therefor
165263, A01N 102
Patent
active
058637150
ABSTRACT:
A method for bulk cryopreservation of biological material includes the steps of providing a flexible container, such as a freezer bag, containing biological material that is treated with a cryoprotectant and freezing the biological material to below -100.degree. C. and preferably below -196.degree. C. for deep freeze long-term storage. In the preferred embodiment, the bag is placed in a holder that maintains the cross-sectional area of the bag essentially constant and small enough (e.g., about 5 mm width to facilitate uniform heat transfer to and from all regions the bag. This facilitates uniform nucleation of the biological material after supercooling which enables controlled and uniform slow cooling through from about -7.5.degree. C. to a temperature in the range of about -40.degree. C. to -80.degree. C., thereby maintaining the viability of the cells. The single freezer bag method facilitates cryopreservation of large amounts of material, such as entire preparations of isolated islets, as compared to conventional multiple tube cryopreservation protocols and yields similar or improved recovery. In addition, the single freezer bag method reduces the risk of cross-contamination, a problem when adding and withdrawing material form multiple tubes before, during and after the cryopreservation freeze-thaw cycle. The bag construction also reduces the risk of freezing medium (e.g., liquid nitrogen) seeping into the bag and causing the bag to burst when thawing the material. The present invention is directed, in particular, to the cryopreservation of encapsulated islets in a flexible container wherein the container thickness during freezing is maintained around 5 mm. The freezing may also be accomplished by supercooling the encapsulated islets and inducing ice nucleation.
REFERENCES:
patent: 3943770 (1976-03-01), McDonald
patent: 4117881 (1978-10-01), Williams et al.
patent: 4194369 (1980-03-01), Faust et al.
patent: 4251995 (1981-02-01), Pert et al.
patent: 4327799 (1982-05-01), Scheiwe et al.
patent: 4352883 (1982-10-01), Lim
patent: 4386895 (1983-06-01), Sodickson
patent: 4469227 (1984-09-01), Faust
patent: 4565073 (1986-01-01), Lavender
patent: 4675140 (1987-06-01), Sparks
patent: 4692284 (1987-09-01), Braden
patent: 4764463 (1988-08-01), Mason
patent: 4789550 (1988-12-01), Hommel et al.
patent: 4814274 (1989-03-01), Shioya et al.
patent: 4956128 (1990-09-01), Hommel et al.
patent: 4965186 (1990-10-01), Grischenko et al.
patent: 5017338 (1991-05-01), Surgenor
patent: 5071741 (1991-12-01), Brockbank
patent: 5175093 (1992-12-01), Seifert
patent: 5192553 (1993-03-01), Boyse et al.
patent: 5364756 (1994-11-01), Livesey et al.
Rajotte, R.V. et al., "Pancreatic Islet Banking the Transplantation of Frozen Thawed Rat Islets Transported Between Centers," Abstract, AN 82:183249 BIOSIS.
Rajotte, R.V. et al., "Pancreatic Islet Banking the Transplantation of Frozen Thawed Rat Islets Transported Between Centers," Cryobiology, 18:357-369 (1981).
Brendel et al., "Improved functional survival of human islets of langerhans in three-dimensional matrix culture," Cell Transplantation, 3(5):427-435 (1994).
Chao et al., "Entrapment of cultured pancreas islets in three-dimensional collagen matrices," Cell Transplantation, 1:51-60 (1992).
Coulombe et al., "Prolongation of islet xenograft survival by cryopreservation," Diabetes, 36(9):1086-1088 (1987).
Evans et al., "Reversal of diabetes in dogs by transplantation of pure cryopreserved islets," Transplantation, 50(2):202-206 (1990).
Lacy et al., "Perifusion of isolated rat islets in vitro--Participation of the microtubular system inthe biphasic release of insulin," Diabetes, 21:987-998 (1972).
Lakey et al., "Development of a method for bulk cryopreservation of purified canine islets and human pancreatic microfragments," Cell Transplantation, 3:213 (1994).
Ohgawara et al., "Maintenance of embedded pig pancreatic pseudo-islets in a collagen gel matrix: study of the effect of hydrocortisone, a collagenase inhibitor, and nicotinamide on collagenolysis and the morphogenesis of pancreatic islet-cells in collagen gel matrix," In Vitro Cell. Dev. Biol., 26:348-352 (1990).
Rajotte et al., "Islet isolation and cryopreservation in rats and dogs," Diabetes Mellitus, pp. 218-228 (1990).
Rajotte et al., "Viability studies on frozen-thawed rat islets of langerhans," Cryobiology, 14:116-120 (1977).
Rajotte et al., "Feasibility of low temperature banking for pancreatic islet cell transplantation," Islet Isolation, Culture and Cryopreservation, pp. 124-133 (1981).
Rajotte et al., "Transplantation of cryopreserved and fresh rat islets and canine pancreatic fragments: comparison of cryopreservation protocols," Cryobiology, 20:169-184 (1983).
Rajotte et al., "Cryopreservation of insulin-producing tissue in rats and dogs," World J. Surg., 8:179-186 (1984).
Rajotte et al., "Islet cryopreservation for clinical trials," Diab. Nutr. Metab., 5(1):63-68 (1992).
Rajotte et al., "Optimizing Cryopreservation of isolated islets," Transplantation Proceedings, 21(1):2638-2640 (1989).
Rajotte et al., "Methods of islet cryopreservation," Pancreatic Islet Cell Transplantation, pp. 124-131 (1992).
Ricordi et al., "Islet isolation assessment in man and large animals," Acta Diabetol. Lat., 27:185-195 (1990).
Colby C. Budd
Flashner Michael
Lakey Jonathan R.T.
Rajotte Ray V.
Warnock Garth L.
Saucier Sandra E.
The Governors of the University of Alberta
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