Liquid purification or separation – Processes – Using magnetic force
Patent
1997-06-02
2000-01-11
Reifsnyder, David A.
Liquid purification or separation
Processes
Using magnetic force
210745, 209214, 2092231, 435 72, 436172, 436177, 436526, B01D 3506, G01N 33533
Patent
active
060131888
ABSTRACT:
A method of qualitative analysis of microscopic biological specimens is disclosed in which the specimens are rendered magnetically responsive by immunosspecific binding with feuromagnetic colloid. The specimens are placed in a vessel having a feuromagnetic capture structure supported on a transparent wall. The vessel is then placed in a magnetic field for inducing a high gradient region in the vicinity of the capture structure and, in particular, adjacent to the capture structure along the direction of the applied field. The magnetically responsive specimens are collected and immobilized by the high gradient along the wall adjacent to the capture structure for unobstructed microscopic observation. Subpapulations of distinct types of cells can thus be observed and differentiated. As transport force, such as gravity or an externally applied magnetic gradient, may be applied to the vessel to move the specimens into the high gradient region.
REFERENCES:
patent: 4729949 (1988-03-01), Weinreb et al.
patent: 4735504 (1988-04-01), Tycko
patent: 4989978 (1991-02-01), Groner
patent: 5030560 (1991-07-01), Sinor et al.
patent: 5053344 (1991-10-01), Zborowski et al.
patent: 5200084 (1993-04-01), Liberty et al.
patent: 5340749 (1994-08-01), Fujiwara et al.
patent: 5375606 (1994-12-01), Slezak et al.
patent: 5411863 (1995-05-01), Miltenyi
patent: 5428451 (1995-06-01), Lea et al.
patent: 5451525 (1995-09-01), Shenkin et al.
patent: 5466574 (1995-11-01), Liberti et al.
patent: 5494831 (1996-02-01), Kindler
patent: 5498550 (1996-03-01), Fujiwara et al.
patent: 5541072 (1996-07-01), Wang et al.
patent: 5646001 (1997-07-01), Terstappen et al.
Chen, et al., "Automated Enumeration of CD34+Cells in Peripheral Blood and Bone Marrow", J. of Hematotherapy; 3:3-13 (1994).
de Groth, et al., "The Cytodisk: A Cytometer Based Upon a New Principle of Cell Alignment", Cytometry; 6:226-233 (1995).
Kamentsky, et al., "Microscope-Based Muliparameter laser scanning Cytometer Yielding Data Comparable to Flow Cytometry Data", Cytometry: 12:381-387 (1991).
Stewart, et al., "Quantitation of Cell Concentration Using the Flow Cytometer", Cytometry; 2:238-243 (1992).
Takayasu, et al., "HGMS Studies of Blood Cell Behavior in Plasma", IEEE Transactions of Magnetics, 18:1520-1522 (1982).
Takayasu, et al., "High Gradient Magnetic Seperation II. Single Wire Studies of Shale Oils", IEEE transactions on Magnetics; 18:1695-1697 (1982).
Zwerner, et al., "A Whole Blood Alternative to Traditional Methods for CD4+T Lymphocyte Determine" J. of Acquired immune Deficiency Syndromes and Human Retrovirology; 14:31-34 (1997).
Liberti Paul A.
Terstappen Leon W. M. M.
Immunivest Corporation
Reifsnyder David A.
LandOfFree
Methods for biological substance analysis employing internal mag does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for biological substance analysis employing internal mag, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for biological substance analysis employing internal mag will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1459240