Methods for automated synthesis of PNA-DNA chimeras and composit

Details Methods for automated synthesis of PNA-DNA chimeras and composit Methods for automated synthesis of PNA-DNA chimeras and composit

1997-08-11

2000-05-16

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 221, 536 231, 536 253, 536 2532, 536 2533, 536 2534, C12Q 168, C07H 1900, C07H 2100, C07H 2102

Patent

active

06063569&

ABSTRACT:
A method is disclosed for the preparation of novel PNA synthons compatible with DNA synthetic reagents and instrumentation. Accordingly, the PNA synthons of this invention are particularly suitable for the preparation of PNA-DNA chimeras, among other oligomers. The PNA synthons are designed to have a protecting group strategy which is orthogonal and allows removal of the protecting groups under mild conditions. Generally, an acid labile protected backbone is coupled to a nucleobase side chain moiety to form the PNA synthon. A novel method for synthesizing the acid labile protected backbone also is described. In addition, novel compositions of matter are disclosed.

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