Methods for attachment of a polynucleotide to a preselected mate

Details Methods for attachment of a polynucleotide to a preselected mate Methods for attachment of a polynucleotide to a preselected mate

1995-12-19

2000-02-08

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 912, 435 913, 435 915, 435 9151, 435 9152, 435810, 436501, 536 221, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 536 253, 935 16, 935 17, 935 77, 935 78, 935 88, C07H 2100, C07H 2102, C07H 2104, C12P 1934

Patent

active

060227141

ABSTRACT:
Compositions and methods for selectively linking a polynucleotide through its 5' or 3' end to one or more preselected materials such as insoluble matrices, solid supports, proteins, small molecular or labels are disclosed. Use of these compositions and methods in the production of diagnostic and affinity reagents are also disclosed.

REFERENCES:
patent: 4711955 (1987-12-01), Ward et al.
patent: 4828979 (1989-05-01), Klevan et al.
Smith et al. (1985) Nucleic Acid Research, vol. 13, No. 7, pp. 2399-2412.

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