Methods for attaching ligands or anti-ligands to a solid phase

Details Methods for attaching ligands or anti-ligands to a solid phase Methods for attaching ligands or anti-ligands to a solid phase

1987-05-08

1988-12-13

Warden, Robert J.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

436534, 436535, 436807, 436809, 427 2, G01N 33531

Patent

active

047910690

ABSTRACT:
Methods for attaching one component of a ligand-anti-ligand pair onto a solid phase surface. The component may be attached to a photoactivatable cross-linker capable of coupling the component to a colloidal medium coating the surface or alternatively, the component may be coupled to a bead and held in place against the surface by an overlay of the colloidal media having voids and spaces smaller than the diameter of the particle but large enough to permit diffusion of the other of the ligand-anti-ligand pair to be detected.

REFERENCES:
patent: 3074853 (1963-01-01), Brewer
patent: 4338094 (1982-07-01), Elahi
patent: 4487839 (1984-12-01), Kamentsky
patent: 4672024 (1987-06-01), Giaever
patent: 4748042 (1988-05-01), Linnecke

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