Methods for assessing risk for cardiac dysrythmia in a human...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200, C536S023100, C536S023500, C536S024310, C536S024330

Reexamination Certificate

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10475452

ABSTRACT:
The present invention relates to methods for assessing the risk of a patient for developing a potentially fatal cardiac dysrhythmia and for diagnosing Andersen's Syndrome. A tissue sample from a patient is obtained and the DNA or proteins of the sample isolated. From the DNA and protein isolates the sequence of the KCNJ2 gene or the Kir2.1 polypeptide can be obtained. The KCNJ2 gene or the Kir2.1 can be screened for alteration as compared to the wile-type sequence. An alteration in a copy of the KCNJ2 gene or a Kir2.1 polypeptide indicates that the patient has a high risk for developing a cardiac dysrhythmia and can be diagnosed with Andersen's Syndrome. The invention also related to isolated nucleic acid molecules with one or more alterations as compared to the wild-type sequence.

REFERENCES:
patent: 6274332 (2001-08-01), Keating et al.
Ptacek et al. American Journal of Human Genetics. Oct. 2001. 69: p. 230, Abstract #275.
NCBI printout of SNPs in the KCNJ2 gene. National Center for Biotechnology, National Library of Medicine, NIH (Bethesda, MD, USA), 2006.
Davies et al. Neurology. 2005. 65: 1083-1089.
Plaster et al. May 2001. Cell. 105: 511-519.
Bendahhou, Said, et al., “Defective Potassium Channel Kir2.1 Trafficking Underlies Andersen-Tawil Syndrome,” The Journal of Biological Chemistry, vol. 278, No. 51, Dec. 19, 2003, pp. 51779-51785.
Donaldson, M.R., et al., “PIP2Binding Residues of Kir2.1 are Common Targets of Mutations causing Andersen Syndrome,” Neurology 60, Jun. 2003, pp. 1811-1816.
Tristani-Firouzi, Martin, et al., Functional and Clinical Characterization of KCNJ2 Mutations Associated with LQT7 (Andersen Syndrome), The Journal of Clinical Investigation, Aug. 2002, vol. 110, No. 3, pp. 381-388.
Kubo, et al., “A Mutation of the Cloned Inward Rectifier K+ Channel, IRK1, which causes a Slowdown of the Activation and a Negative Shift of the Conductance Curve,” Japanese Journal of Physiology. 1995, vol. 45, Supplement 1, p. S118, abstract 325.
Mylona, et al., “Detection of a High-Frequency Silent Polymorphin (C to T) in the Kir2.1 (KCNJ2) inwardly Rectifying Potassium Channel Gene by Polymerase Chain Reaction and Single Strand Conformation Polymorphism.” Molecular and Cellular Probes, 1998, vol. 12, pp. 331-333, especially p. 332.
Splawski, et al., “Mutations in KCNJ2, an inward Rectifier Potassium Channel Gene, cause Andersen's Syndrome.” American Journal of Human Genetics. Oct. 2001, vol. 69, Supplement 2, p. 230, abstract 276.

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