Methods for assessing 1,25(OH).sub.2 D.sub.3 activity in skin an

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – 9,10-seco- cyclopentanohydrophenanthrene ring system doai

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514255, 514560, A61K 3159

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active

059983930

ABSTRACT:
Described are in vivo methods for testing the activity of vitamin D.sub.3 (1,25-dihydroxyvitamin D.sub.3) and analogs thereof in human skin and for testing the effect of a test compound on said activity. Also described are methods for enhancing the in vivo activity of vitamin D.sub.3 and analogs thereof by coadministering vitamin D.sub.3 or an analog thereof with an RXR ligand, a 24-hydroxylase inhibitor, or a mixture thereof. Preferred RXR ligand materials are retinoids, such as 9-cis retinoic acid, and precursors thereof, such as all trans retinoic acid. Preferred 24-hydroxylase inhibitors are imidazoles, especially those inhibiting cytochrome-P450 oxidative enzymes, such as ketoconazole. Also included is a method for determining whether a candidate compound inhibits 24-hydroxylase activity or induction thereof.

REFERENCES:
patent: 4569935 (1986-02-01), Rosenberg et al.
Spearman et al., Brit. J. Dermatol., 90(5), 553-60 (Abstract), 1974.
Lutzow-Holm et al. Experimental Dermatology, 2(3) 113-20 (Denmark) (Abstract), 1993.

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