Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-11-01
2005-11-01
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200
Reexamination Certificate
active
06960434
ABSTRACT:
Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.
REFERENCES:
patent: 6251594 (2001-06-01), Gonzalgo et al.
patent: WO 00/04187 (2000-01-01), None
patent: WO 00/26401 (2000-05-01), None
Labosky, P.A. et al., “Mouse embryonic germ (EG) cell lines: transmission through the germline and differences in the methylation imprint of insulin-like growth factor 2 receptor (Igf2r) gene compared with embryonic stem (ES) cell lines”,Development,vol. 120, pp. 3197-3204 (1994).
Matsui, Y. et al., “Derivation of Pluripotential Embryonic Stem Cells from Murine Primordial Germ Cells in Culture”,Cell,vol. 70, pp. 841-847 (1992).
Mitsuya, K. et al., “Epigenetic reprogramming of the human H19 gene in mouse embryonic cells does not erase the primary parental imprint”,Genes to Cells,vol. 3, pp. 245-255 (1998).
Ogawa, O. et al., “Relaxation of insulin-like growth factor II gene imprinting implicated in Wilms' tumour”,Nature,vol. 362, pp. 749-751 (1993).
Overall, M. et al., “Genomic Imprinting in the Rat: Linage of Igf2 and H19 Genes and Opposite Parental Allele-Specific Expression During Embryogenesis”,Genomics,vol. 45, pp. 416-420 (1997).
Sutcliffe, J.S. et al., “Deletions of a differentially methylated CpG island at the SNRPN gene define a putative imprinting control region”,Nature Genetics,vol. 8, pp. 52-58 (1994).
Hayward, B.E et al., “Bidirectional Impriting of a single gene: GNAS1 encodes maternally, paternally, and biallelically derived proteins”,Proc. Natl. Acad. Sci.,vol. 95, pp. 15475-15480 (1998).
Lee, C.K. et al., “Isolation and Genetic Transformation of Primordial Germ Cell (PGC)—Derived Cells from Cattle, Goates, Rabbits and Rats”,Asian-Australasian Journal of Animal Sciences,vol. 13, No. 5, pp. 587-594 (2000).
Gregorova, S. et al., “PWD/Ph and PWK/Ph inbred mouse strains of Musm. musculussubspecies: A valuabl3e resource of phenotypic variations and genomic polymorphisms”,Folia Biologica,vol. 46, No. 1, pp. 31-42 (2000) ABSTRACT.
Uejima, H. et al., Hot-stop PCR: a simple and general assay for linear quantitation of allele ratios,Nature Genetics,25, Aug. 2000, pp. 375-376.
Feinberg, “Methylation meets genomics”,Nature Genetics,27, Jan. 2001, pp. 9-10.
Feinberg Andrew P.
Jiang Shan
Strichman-Almashanu Liora
DLA Piper Rudnick Gray Cary US LLP
Horlick Kenneth R.
The Johns Hopkins University
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