Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Reexamination Certificate
2011-08-30
2011-08-30
Nguyen, Bao Thuy L (Department: 1641)
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
C435S007100, C435S007920, C435S007940, C435S007950, C435S283100, C435S288300, C435S288700, C435S970000, C436S527000
Reexamination Certificate
active
08008094
ABSTRACT:
As a result of investigating the optimum conditions of methods for immobilizing proteins that interact with sugar chains onto a substrate, it was revealed that coating the surface of a slide glass with GTMS enables immobilization at a higher S/N ratio than conventionally possible. Moreover, by using a substrate to which a rubber with a number of holes was affixed to form a number of reaction vessels, and further by spotting lectins onto the substrate and washing with PBST, the weak interactions between sugar chains and lectins were successfully detected with improved sensitivity. In addition, by introducing an evanescent excitation-type scanner, it became possible to detect the interactions between lectins and sugar chains without washing away the probe solution.
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Hirabayashi Jun
Kuno Atsushi
Nakamura Sachiko
Uchiyama Noboru
Klarquist & Sparkman, LLP
National Institute of Advanced Industrial Science and Technology
Nguyen Bao Thuy L
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