Methods for amplifying polymeric nucleic acids

Details Methods for amplifying polymeric nucleic acids Methods for amplifying polymeric nucleic acids

2004-08-02

2009-12-08

Kim, Young J (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C536S024330

Reexamination Certificate

active

07629152

ABSTRACT:
The invention provides compositions and methods for amplifying nucleic acid polymer sequences in a high complexity nucleic acid sample. The unique compositions of the invention include a primer set composed of a mixture of two types of primers for DNA synthesis. For extension in one direction, the primers all contain modifications that destroy their ability to serve as templates that can be copied by DNA polymerases. For extension in the opposite direction the set includes at least one primer that can serve as a template and be replicated by DNA polymerases throughout its length. The method can be carried out by mixing the nucleic acid polymer sequence of interest with the set of DNA synthesis primers in an amplification reaction mixture. The reaction mixture is then subjected to temperature cycling analogous to the temperature cycling in PCR reactions. At least one primer in the primer set hybridizes to the nucleic acid polymer. It is preferred that the non-replicable primer hybridizes to the nucleic acid polymer and is extended to produce an extension product that contains sequence from the nucleic acid polymer to which the replicable primer then hybridizes. Of course, if the nucleic acid polymer is double stranded, both the replicable and nonreplicable primers will hybridize and be extended by DNA polymerase.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4957858 (1990-09-01), Chu et al.
patent: 5219727 (1993-06-01), Wang et al.
patent: 5422252 (1995-06-01), Walker et al.
patent: 5455166 (1995-10-01), Walker
patent: 5683896 (1997-11-01), Hartley et al.
patent: 5824517 (1998-10-01), Cleuziat et al.
patent: 5998583 (1999-12-01), Korsmeyer
patent: 6027923 (2000-02-01), Wallace
patent: 6124120 (2000-09-01), Lizardi
patent: 6214587 (2001-04-01), Dattagupta et al.
patent: 6251639 (2001-06-01), Kurn
patent: 6335184 (2002-01-01), Reyes et al.
patent: 7112406 (2006-09-01), Behlke et al.
patent: 2002/0072095 (2002-06-01), Hartley et al.
patent: 2003/0228596 (2003-12-01), Liu et al.
patent: 0 416817 (1996-10-01), None
patent: 01/64952 (2001-09-01), None
patent: 03/074724 (2003-09-01), None
Stump et al. The use of modified primers to eliminate cycle sequencing artifacts. Nucleic Acids Research, 1999, vol. 27, No. 23, pp. 4642-4648.
Edwards et al. Multiplex PCR: Advantages, Development, and Applications. (PCR Methods and Applications 3:S65-S73, 1994).
Eritja et al., Synthesis of Oligonucleotides Containing the Abasic Site Model Compound 1.4-Anhydro-2-Deoxy-D-Ribitol,Nucleosides&Nucleotides, vol. 6. No. 4, 803-814.
Kwok and Higuchi, Avoiding false positives with PCR,Nature, vol. 339, May 1989, 237-238.
Saiki et al., Enzymatic Amplification of B-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia,Science, vol. 230, 1350-1354.
Newton et al., Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS),Nucleic Acid Research, vol. 17, No. 7, 1989, 2504-2516.
Seela et al., Oligodeoxyribonucleolides containing 1,3-propanediol as nucleoside substiture,Nucleic Acids Research, vol. 15, No. 7, 1987. 3113-3129.
Longo et al., Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions,Gene, 93(1); 1990, 125-128.
Ugozzoli and Wallace, Allele-Specific Polymerase Chain Reaction,Methods, vol. 2, No. 1, Feb. 1991, 42-48.
Reyes et al., Linked Linear Amplification: A New Method for the Amplification of DNA,Clinical Chemistry2001, 47:1, 31-40.
Wu and Wallace, The Ligation Amplification Reaction (LAR)-Amplification of Specific DNA Sequences Using Sequential Rounds of Template-Dependent Ligation,Genomics, vol. 4, 1989, 560-569 .
Newton et al., The Production fo PCR products with 5′ single-stranded tails using primers that incorporate novel phosphoramidite intermediates,Nucleic Acids Research, vol. 21. No. 5, 1993, 1155-1162.
Gade et al., Incorporation of Nonbase Residues into Synthetic Oligonucleotides and Their Use in the PCR,Gata 10(2), 1993, 61-65.
Walder et al., use of PCR primers containing a 3′-terminal ribose residue to prevent cross-contamination of amplified sequences,Nucleic Acids Researchvol. 21, No. 18, 1993, 4339-4343.
Walker et al., Strand displacement amplification—an isothermal, in vitro DNA amplification technique,Nucleic Acids Research, vol. 20, No. 7, 1992, 1691-1966.
Garcia-Quintanilla et al., Single-Tube Balanced Heminested PCR for Detecting Mycobacterium Tuberculosis in Smear-Negative Samples, Journal of Clinical Microbiology, vol. 38, No. 3, 2000 1166-1169.
Little et al., Strand Displacement Amplification and Homogenous real-Time Detection Incorporated in a Second-Generation DNA Probe System, BDProbeTecET,Clinical Chemistry, 45:6, 1999, 777-784.
Nycz et al., Quantitative Reverse Transcription Strand Displacement Amplification: Quantitation of Nucleic Acids Using an Isothermal Amplification Technique, Analytical Biochemistry, 259, 1998, 226-234.
Lizardi et al., Mutation Detection and Single-Molecule Counting using Isothermal Rolling-Circle Amplification,Nature Genetics, vol. 19, 1998, 225-232.
Poddar, Symmetric vs Asymmetric PCR and Molecular Beacon Probe in the Detection of a Target Gene of Adenovirus,Molecular and Cellular Probes, 14, 2000, 25-32.

Affiliated with

Also associated with

Rating

Methods for amplifying polymeric nucleic acids does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for amplifying polymeric nucleic acids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for amplifying polymeric nucleic acids will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4097695