Methods for amplification of nucleic acid sequences using...

Details Methods for amplification of nucleic acid sequences using... Methods for amplification of nucleic acid sequences using...

2004-05-07

2009-02-24

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S091200

Reexamination Certificate

active

07494789

ABSTRACT:
Methods and kits are provided for producing sense RNA molecules. The sense RNA molecules are prepared by:providing a single stranded cDNA molecule having 5′ and 3′ ends;attaching an oligodeoxynucleotide tail to the 3′ end of said single stranded cDNA molecule;providing a double stranded RNA polymerase promoter having a sense strand and antisense strand, wherein the sense strand comprises a single stranded 3′ overhang comprising a sequence complementary to said oligodeoxynucleotide tail;annealing said double stranded RNA polymerase promoter to said oligodeoxynucleotide tail by complementary base pairing with said 3′ overhang sequence;ligating the 5′ end of the antisense strand of said double stranded RNA polymerase promoter to the 3′ end of said oligodeoxynucleotide tail; andinitiating RNA transcription using an RNA polymerase which recognizes said double stranded promoter, thus producing a sense RNA molecule (sRNA).

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Rajeevan M S et al, Genomics, Academic Press, San Diego, 82:4:491-497 (2003).
Lawson Jonathan N et al, DNA and Cell Biology, 25:11:627-634 (2006).

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