Methods for altering surface characteristics of microspheres

Chemistry: analytical and immunological testing – Optical result – Including reagent preparation

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C436S056000, C424S490000, C536S053000, C536S055300, C435S395000, C435S396000

Reexamination Certificate

active

07867774

ABSTRACT:
Various methods for altering surface characteristics of a microsphere are provided. One method includes coupling an enolic acid to the microsphere to modify the surface characteristics of the microsphere. The surface characteristics may include charge density and/or pKa. A reagent can be coupled to the microsphere via the enolic acid. The reagent may include a biomolecule. The modified surface characteristics may increase a stability of the reagent when the reagent is coupled to the microsphere. The modified surface characteristics may also improve performance of an assay carried out with the microsphere. Another embodiment relates to a microsphere that includes an enolic acid coupled to a polymer core of the microsphere such that the enolic acid modifies surface characteristics of the microsphere. A reagent can be coupled to the microsphere via the enolic acid.

REFERENCES:
patent: 2657205 (1953-10-01), Heyna et al.
patent: 4935147 (1990-06-01), Ullman et al.
patent: 5414135 (1995-05-01), Snow et al.
patent: 5674699 (1997-10-01), Saunders et al.
patent: 5736330 (1998-04-01), Fulton
patent: 5763203 (1998-06-01), Ugelstad et al.
patent: 5981180 (1999-11-01), Chandler et al.
patent: 6008406 (1999-12-01), Stolowitz
patent: 6046807 (2000-04-01), Chandler
patent: 6057107 (2000-05-01), Fulton
patent: 6075126 (2000-06-01), Stolowitz et al.
patent: 6124471 (2000-09-01), Stolowitz et al.
patent: 6139800 (2000-10-01), Chandler
patent: 6146833 (2000-11-01), Milton
patent: 6268222 (2001-07-01), Chandler et al.
patent: 6366354 (2002-04-01), Chandler
patent: 6411904 (2002-06-01), Chandler
patent: 6449562 (2002-09-01), Chandler et al.
patent: 6462179 (2002-10-01), Stolowitz et al.
patent: 6514295 (2003-02-01), Chandler et al.
patent: 6524793 (2003-02-01), Chandler et al.
patent: 6602692 (2003-08-01), Glusenkamp et al.
patent: 6630577 (2003-10-01), Stolowitz et al.
patent: 6632526 (2003-10-01), Chandler et al.
patent: 6656876 (2003-12-01), Åberg et al.
patent: 2003/0028981 (2003-02-01), Chandler et al.
patent: 2004/0039201 (2004-02-01), Lugade et al.
patent: 2004/0087032 (2004-05-01), Chandler et al.
patent: 2005/0118199 (2005-06-01), Esser et al.
patent: 960534 (1957-03-01), None
patent: 0230768 (1992-03-01), None
patent: 8092294 (1996-04-01), None
patent: 99/67228 (1999-12-01), None
patent: 02/40698 (2002-05-01), None
Kaminski, “Triazine-Based Condensing Reagents”, Peptide Science, vol. 55, No. 2, pp. 140-164 (2000) (Kaminski).
International Search Report, PCT/US2005/036264, mailed Jan. 15, 2007.
Pyzhov et al., “Chemical Heterocyclic Connections,” © VINITI 1982, 8 pages.
Fulton et al., “Advanced multiplexed analysis with the FlowMetrix™ system,” Clinical Chemistry, vol. 43, No. 9, 1997, pp. 1749-1756.
Adams et al., “Far-infrared Reflectance Spectra of Some Hexachlorotellurates and Other Hexachlorometallates,” J. Chem. Soc. (A), 1971, pp. 878-879.
Ahne et al., “Partielle Aminolyse von 2,4,6-Triallyloxy-s-triazin. Teil 2: Herstellung von N,N′-Bis[4,6-diallyloxy-2-s-triazinyl]diaminoalkanen,” Synthesis Communications, Mar. 1975, pp. 184-186.
Bartels-Keith et al., “A Synthesis of Stipitatic Acid,” J. Chem. Soc., 1951, pp. 2352-2356.
Bellus, “[2+2] Cycloadditions of Tetraalkoxyethylenes with Ketenes: a General Route to 2-Substituted 1-Hydroxycyclobut-1-ene-3,4-diones,” Journal of the American Chemical Society, vol. 100, No. 25, Dec. 1978, pp. 8026-8028.
Belogi et al., “Polystyrylboronic acid as a reusable polymeric support for oligosaccharide synthesis,” Tetrahedron Letters, No. 41, 2000, pp. 6965-6968.
Hermanson,Bioconjugate Techniques, © Elsevier 1996, Chapters 1-9.
Hermanson,Bioconjugate Techniques, © Elsevier 1996, Chapters 10-17.
Blixt et al., “Enzymatic glycosylation of reducing oligosaccharides linked to a solid phase or a lipid via a cleavable squarate linker,” Carbohydrate Research, vol. 319, 1999, pp. 80-91.
Cole et al., “Toxin from Fusarium moniliforme: Effects on Plants and Animals,” Science, vol. 179, 1973, pp. 1324-1326.
Frey et al., “Grafting Protein Ligand Monolayers onto the Surface of Microparticles for Probing the Accessibility of Cell Surface Receptors,” Bioconjugate Chem., vol. 10, 1999, pp. 562-571.
James et al., “Novel Photoinduced Electron-transfer Sensor for Saccharides based on the Interaction of Boronic Acid and Amine,” J. Chem. Soc., Chem. Commun., 1994, pp. 477-478.
Konek et al., “Interfacial Acidities, Charge Densities, Potentials, and Energies of Carboxylic Acid-Functionalized Silica/Water Interfaces Determined by Second Harmonic Generation,” J. Am. Chem. Soc., vol. 124, 2004, pp. 11754-11755.
Kunishima et al., “Synthesis and Characterization of 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium Chloride,” Tetrahedron Letters, No. 40, 1999, pp. 5327-5330.
Law et al., “Squaraine Chemistry. Synthesis, Characterization, and Optical Properties of a Class of Novel Unsymmetrical Squaraines: [4-(Dimethylamino)phenyl](4′-methoxyphenyl)squaraine and Its Derivatives,” J. Org. Chem., vol. 57, 1992, pp. 3278-3286.
Kettman et al., “Classification and Properties of 64 Multiplexed Microsphere Sets,” Cytometry, vol. 33, 1998, pp. 234-243.
Meier et al., “Bis(stilbenyl)squaraines—Novel Pigments with Extended Conjugation,” Tetrahedron Letters, vol. 37, No. 8, 1996, pp. 1191-1194.
Roberts et al., “Chemistry for peptide and protein PEGylation,” Advanced Drug Delivery Reviews, vol. 54, 2002, pp. 459-476.
Salvino et al., “Polymer-Supported Tetrafluorophenol: A New Activated Resin for Chemical Library Synthesis,” J. Comb. Chem., vol. 2, 2000, pp. 691-697.
Schmidt et al., “Die preparative Chemie der Cyclobutendione; I. Synthese con Cyclobutendion und dessen Alkyl-, Alkenyl- und Aryl-Derivaten,” © Georg Thieme Publishers 1978, pp. 1-22.
Venkataraman et al., “Cyanuric Chloride: A Useful Reagent for Converting Carboxylic Acids Into Chlorides, Esters, Amides and Peptides,” Tetrahedron Letters, No. 32, 1979, pp. 3037-3040.
Yoon et al., “Fluorescent Chemosensors of Carbohydrates. A Means of Chemically Communicating the Binding of Polyols in Water Based on Chelation-Enhanced Quenching,” J. Am. Chem. Soc., vol. 114, 1992, pp. 5874-5875.
Valuev et al., “Chemical modification of polymers with physiologically active species using water-soluble carbodiimides,” Biomaterials, vol. 19, 1998, pp. 41-43.
Renbaum et al., “Design of Polymeric Immunomicrospheres for Cell Labelling and Cell Separation,” The British Polymer Journal, vol. 10, Dec. 1978, pp. 275-280.
Medvedkin et al., “p-Sulfotetrafluorophenyl Hydrophilic Active Esters of Amino Acids in Peptide Synthesis,” Bioorg. Khirn., vol. 21, No. 9, 1995, pp. 684-690.
Kashkin et al., “Fluorine-Containing Hydroxyalkylamino-1,3,5-Triazines,” Zhurnal Organicheskoi Khimii, vol. 12, No. 9, Sep. 1976, pp. 2030-2033.
Bergseid et al., “Small Molecule-Based Chemical Affinity System for the Purification of Proteins,” BioTechniques, vol. 29, No. 5, 2000, pp. 1126-1133.
Sprenger et al., “Cyclobutenediylium Dyes,” Angew. Chem. Internat. Edit., vol. 7, No. 7, 1968, pp. 530-535.
Martin et al., “Einfache Sythesen für Amino-aroxy-1,3,5-triazine,” Journal f. prakt. Chemie. Band, vol. 323, No. 4, 1981, pp. 694-699.
McDade et al., “True Multiplexed Analysis by Computer-Enhanced Flow Cytometry,” Medical Device & Diagnostic Industry Magazine, Apr. 1997, 5 pages.
Methods in Cell Biology, vol. 42, © Academic Press 1994, pp. 575-595.
Nikiforov et al., “Genetic Bit Analysis: a solid phase method for typing single nucleotide polymorphisms,” Nucleic Acids Research, vol. 22, No. 20, 1994, pp. 4167-4175.
Upson, “Reactive Functional Latex Polymers,” Journal of Polymer Scien

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Methods for altering surface characteristics of microspheres does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for altering surface characteristics of microspheres, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for altering surface characteristics of microspheres will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2742537

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.