Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2005-05-20
2010-11-30
Fronda, Christian L (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S183000, C435S252300, C435S320100, C536S023200
Reexamination Certificate
active
07842479
ABSTRACT:
Methods of increasing cultured cell growth yields and/or protein production from bacterial cell cultures are provided. More particularly, mutant bacterial cells having an alteration in the expression or activity of the cidABC operon, a gene therein, or a homolog or a regulator thereof, and methods for reducing acetic acid/acetate production in cultures are provided, as are methods for increasing cultured cell growth yields and/or protein production employing such cells. Methods for enhancing bacterial cell death and methods for identifying agents that increase the susceptibility of bacteria to cell death are also provided.
REFERENCES:
patent: 5139938 (1992-08-01), Shimizu et al.
patent: 5733723 (1998-03-01), van Eekelen et al.
patent: 6107093 (2000-08-01), Ingram et al.
patent: 6150133 (2000-11-01), Mead et al.
patent: 6348582 (2002-02-01), Black et al.
patent: 6428981 (2002-08-01), Joergensen et al.
patent: 6458557 (2002-10-01), Miller et al.
patent: 6593114 (2003-07-01), Kunsch et al.
patent: 6630328 (2003-10-01), Quax et al.
patent: WO 98/58049 (1998-12-01), None
patent: WO 02/059148 (2002-08-01), None
patent: WO 02/094868 (2002-11-01), None
Chica et al. Curr Opin Biotechnol. Aug. 2005;16(4):378-84.
Sen et al. Appl Biochem Biotechnol. Dec. 2007;143(3):212-23.
Airistidou et al., “Metabolic engineering ofEscherichia colito enhance recombinant protein production through acetate reduction,”Biotechnology Progress11(4) 475-478 (1995) (Abstract Only).
Arbige et al., “Fermentation ofBacillus,” inBacillus subtilis and Other Gram-Positive Bacteria, Hoch et al. (eds.), American Society for Microbiology, Washington, D.C. (1993), pp. 871-873.
Bayles, “Are the molecular stategies that control apoptosis conserved in bacteria?”Trends in Microbiology11(7): 306-311 (2003).
Farmer and Liao, “Reduction of Aerobic Acetate Production byEscherichia coli,” Applied and Environmental Microbiology63(8): 3205-3210 (1997).
Ferrari et al., “Commercial Production of Extracellular Enzymes,” inBacillus subtilis and Other Gram-Positive Bacteria, Hoch et al. (eds.), American Society for Microbiology, Washington, D.C. (1993), pp. 917-931.
Fujimoto et al., “Analysis of Genetic Elements ControllingStaphylococcus aureus lrgABExpression: Potential Role of DNA Topology in SarA Regulation,”Journal of Bacteriology182(17): 4822-4828 (2000).
Juty et al., “TheKlebsiella pneumoniaecytomchromebd' terminal oxidase complex and its role in microaerobic nitrogen fixation,”Microbiology143: 2673-2683 (1997).
March et al. “Expression of an Anaplerotic Enzyme, Pyruvate Carboxylase, Improves Recombinant Protein Production inEscherichia coli,” Applied and Environmental Microbiology68(11): 5620-5624 (2002).
Neubauer et al., “Metabolic load of recombinant protein production: inhibition of cellular capacities for glucose uptake and respiration after induction of a heterologous gene inEscherichia coli,” Biotechnology and Bioengineering83(1): 53-64 (2003) (Abstract Only).
Parry et al.,A Colour Atlas of Bacillus Species, Wolfe Medical Publications Ltd (1983), pp. 239-244.
Patton et al. “TheStaphylococcus aureus cidCgene encodes a pyruvate oxides that affects acetate metabolism and cell death in stationary phase,” Abstract of presentation to American Society of Microbiology, 2004 Annual Meeting, publicly available at least as early as May 23, 2004.
Patton et al. “TheStaphylococcus aureus cidCgene encodes a pyruvate oxides that affects acetate metabolism and cell death in stationary phase,”Molecular Microbiology56(6): 1664-1674 (2005).
Patton et al., “The role of proton motive force in expression of theStaphylococcus aureus cidandlrgoperons,”Molecular Microbiology59(5): 1395-1404 (2006).
Perkins and Pero, “Vitamin Biosythesis,” inBacillus subtilis and Its Closest Relatives: from Genes to Cells, Sonenshein et al. (eds.), pp. 271-281, ASM Press, Washington, D.C., (2002).
Rice and Bayles, “Death's toolbox: examining the molecular components of bacterial programmed cell death,”Molecular Microbiology59(3): 729-738 (2003).
Rice et al., “Acetic Acid Induces Expression of theStaphylococcus aureaus cidABCandlrgABMurein Hydrolase Regulator Operons,”Journal of Bacteriology187(3): 813-821 (2005).
Rice et al., “TheStaphylococcus aureaus cidABOperon: Evaluation of Its Role in Regulation of Murein Hydrolase Activity and Penicillin Tolerance,”Journal of Bacteriology185(8): 2635-2643 (2003).
Rice et al., “Transcription of theStaphylococcus aureaus cidandlrgMurein Hydrolase Regulators Is Affected by Sigma Factor B,”Journal of Bacteriology186(10): 3029-3037 (2004).
Somerville et al., “Correlation of Acetate Catabolism and Growth Yield inStaphylococcus aureaus: Implications for Host-Pathogen Interactions,”Infection and Immunity71(8): 4724-4732 (2003).
Tseng et al., “Effect of Microaerophilic Cell Growth Conditions on Expression of the Aerobic (cyoABCDEandcydAB) and Anaerobic (narGHJI, frdABCD,anddmsABC) Respiratory Pathway Genes inEscherichia coli,” Journal of Bacteriology178(4): 1094-1098 (1996).
Wang et al., “Analysis of Codon Usage Patterns of Bacterial Genomes Using the Self-Organizing Map,”Molecular Biology and Evolution18(5): 792-800 (2001).
Yang et al., “A LysR-Type Regulator, CidR, Is Required for Induction of theStaphylococcus aureaus cidABCOperon,” Abstract of presentation to American Society of Microbiology, 2004 Annual Meeting, publicly available at least as early as May 23, 2004.
Yang et al., “A LysR-Type Regulator, CidR, Is Required for Induction of theStaphylococcus aureaus cidABCOperon,”Journal of Bacteriology187(17): 5893-5900 (2005).
Yang et al., “Characterization of theStaphylococcus aureusCidR regulon: elucidation of a novel role for acetoin metabolism in cell death and lysis,”Molecular Microbiology60(2): 458-468 (2006).
Yang et al., “Genetic and metabolic engineering,”Electronic Journal of Biotechnology1(3): 8 pages (Dec. 15, 1998).
Zamboni and Sauer, “Knockout of the high-coupling cytochromeaa3 oxidase reduces TCA cycle fluxes inBacillus subtilis,” FEMS Microbiology Letters226: 121-126 (2003).
Zuber et al., “Peptide Antibiotics,” inBacillus subtilis and Other Gram-Positive Bacteria, Hoch et al. (eds.), American Society for Microbiology, Washington, D.C. (1993), pp. 897-900.
Accession No. AY581892, NCBI DNA Sequence: “Staphylococcus aureusCidR (cidR), CidA (cidA), CidB (cidB), and CidC (cidC) genes, complete cds,” (Apr. 19, 2004) (4 pages).
Bayles Kenneth W.
Patton Toni G.
Rice Kelly C.
Fronda Christian L
Klarquist Sparkman LLP.
The Board of Regents of the University of Nebraska
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