Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium
Patent
1997-01-24
1999-11-09
Benzion, Gary
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Via agrobacterium
800278, 435468, 435419, 4353201, C12N 1500, C12N 1505, C12N 1584, A01H 500
Patent
active
059818409
ABSTRACT:
The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.
REFERENCES:
patent: 5164310 (1992-11-01), Smith et al.
patent: 5177010 (1993-01-01), Goldman et al.
patent: 5384253 (1995-01-01), Krzyzek et al.
patent: 5591616 (1997-01-01), Hiei et al.
Shillito, R.D., Carswell, G.K., and Kramer, C., "Maize Protoplast Culture" in The Maize Handbook, Springer-Verlag, NY, pp. 696 line 2 (1994).
Ritchie et al., "Agrobacterium tumefaciens-mediated expression of gusA in maize tissues", Transgenic Research 2, 252-265 (1993).
Songstad et al., "Establishment of friable embryogenic (type II) callus from immature tassels of zea mays (poaceae)", American Journal of Botany 79-7, 761-764 (1992).
Maize Genetics Cooperative Newsletter (No. 70), 63 (1996).
R.L. Phillips et al., "Cell/Tissue Culture and In Vitro Manipulation" in Corn and Corn Improvement, Third Edition; Sprague et al., eds.; American Society of Agronomy, Inc., Crop Science Society of America, Inc., Soil Science Society of America, Inc.: Madison, WI; pp. 345-387 (1988).
G. Donn et al., "Stable transformation of Maize with a chimaeric, modified Phosphinothricin-acetyltransferase gene from Streptomyces viridochromogenes", Abstracts of the VIIth International Congress on Plant Cell and Tissue Culture, Abstract #A2-38, p. 53 (1990).
An et al., "Functional Analysis of the 3' Control Region of the Potato Wound-Inducible Proteinase Inhibitor II Gene", The Plant Cell, 1, 115-122 (1989).
Armstrong et al., "Development and availability of germplasm with high Type II culture formation response" Maize Genetics Cooperation Newsletter, 65, 92-93 (1991).
Armstrong et al., "Genetic control of plant regeneration from maize tissue cultures", Maize Genetics Cooperation Newsletter, 59, 92-93 (1985).
Bytebier et al., "T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis", Proc. Natl. Acad. Sci. USA, 84 5345-5349 (1987).
Chih-ching, "The N.sub.6 Medium and its Applications to Anther Culture of Cereal Crops"; Proc. Symp. Plant Tissue Culture; Science Press: Peking, pp. 43-50 (1978).
Chilton, "Agrobacterium gene transfer: Progress on a `poor man's vector` for maize", Proc. Natl. Acad. Sci. USA, 90, 3119-3120 (1993).
Christensen et al., "Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation", Plant Mol. Biol., 18, 675-689 (1992).
Ditta et al., "Broad host range DNA cloning system for Gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti", Proc. Natl. Acad. Sci. USA, 77, 7347-7351 (1980).
Dennehey et al., "Comparison of selective agents for use with the selectable maker gene bar in maize transformation", Plant Cell, Tissue and Organ Culture, 36, 1-7 (1994).
Dennis et al., "Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize", Nucleic Acids Research, 12, 3983-4000 (1984).
Duncan et al., "The production of callus capable of plant regeneration from immature embryos of numerous Zea mays genotypes", Planta, 165, 322-332 (1985).
Gallie et al., "The 5'-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo", Nucl. Acids Research, 15, 3257-3273 (1987).
Gardner et al., "The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing", Nucl. Acids Research, 9, 2871-2888 (1981).
Gould et al., "Transformation of Zea mays L. Using Agrobacterium tumefaciens and the Shoot Apex", Plant Physiol., 95 426-434 (1991).
Green et al., "Plant Regeneration from Tissue Cultures of Maize", Crop Sci., 15 417-421 (1976).
Grimsley et al., "Agrobacterium-mediated delivery of infectious maize streak virus into maize plants", Nature, 325, 177-179 (1987).
Herrera-Estrella et al., "Chimeric genes as dominant selectable markers in plant cells", EMBO J., 2, 987-995 (1983).
Hood et al., "T-DNA and Opine Synthetic Loci in Tumors Incited by Agrobacterium tumefaciens A281 on Soybean and Alfalfa Plants", J. Bacteriol., 168, 1283-1290 (1986).
Hood et al., "Restriction Endonuclease Map of pTi Bo542, A Potential Ti Plasmid Vector for Genetic Engineering of Plants", BioTechnology, 702-709-(1984).
Hooykaas, "Transformation of plant cells via Agrobacterium", Plant Mol. Bio., 13, 327-336 (1989).
Horsch et al., "Inheritance of Functional Foreign Genes in Plants", Science, 223, 496-498 (1984).
Ishida et al., "High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens", Nature Biotech., 14, 745-750 (1996).
Jefferson et al., ".beta.-Glucuronidase from Escherichia coli as a gene-fusion marker", Proc. Natl. Acad. Sci. USA, 83, 8447-8451 (1986).
Jin et al., "Genes Responsible for the Supervirulence Phenotype of Agrobacterium tumefaciens A281", J. Bacteriol., 169, 4417-4425 (1987).
Kamo et al., "Regeneration of Zea Mays L. From Embryogenic Callus", Bot. Gaz., 146, 327-334 (1985).
Komari et al., "Physical and Functional Map of Supervirulent Agrobacterium tumefaciens Tumor-Inducing Plasmid pTiBo542", J. Bacteriol., 166, 88-94 (1986).
Komari et al., "Transformation of cultured cells of Chenopodium quinoa by binary vectors that carry a fragment of DNA from the virulence region of pTiBo542", Plant Cell Reports, 9, 303-306 (1990).
Komari et al., "Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers", The Plant Journal, 10, 165-174 (1996).
McCabe et al., "Stable Transformation of Soybean (Glycine Max) by Particle Acceleration", Bio/Technology, 6, 923-926 (1988).
Moloney et al., "Transformation and Foreign Gene Expression" in Monographs Theoretical and Applied Genetics (19); Frankel et al., Eds.; Springer-Verlag: NY; pp. 148-167 (1993).
Morocz et al., "An improved system to obtain fertile regenerants via maize protoplasts isolated from a highly embroyogenic suspension culture", Theor. Appl. Genet., 80, 721-726 (1990).
Neuffer, "Growing Maize for Genetic Purposes"; in Maize for Biological Research; Sheridan, Ed.; Plant Molecular Biology Assoc.; pp. 19-30 (1982).
Ohta et al., "Construction and Expression in Tobacco of a .beta.-Glucuronidase (GUS) Reporter Gene Containing an Intron Within the Coding Sequence", Plant Cell Physiol., 31, 805-813 (1990).
Potrykus, "Gene Transfer to Cereals: An Assessment", BioTechnology, 535-542 (1990).
Schafer et al., "T-DNA integration and expression in a monocot crop plant after induction of Agrobacterium", Nature, 327, 529-532 (1987).
Skirvin, "Fruit Crops" in Coning Agricultural Plants Via In Vitro Techniques; Conger, ed.; CRC Press: Boca Raton, FL; pp. 51-140 (1981).
Songstad et al., "Advances in alternative DNA delivery techniques", Plant Cell, Tissue and Organ Culutre, 40, 1-15 (1995).
Songstad et al., "Production of Transgenic Maize Plants and Progeny by Bombardment of Hi-II Immature Embryos", In Vitro Cell, Dev. Biol.-Plant, 32, 179-183 (1996).
Smith et al., "Agrobacterium tumefaciens Transformation of Monocotyledons", Crop Sci., 35, 301-309 (1995).
Thompson et al., "Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus", EMBO J., 6, 2519-2523 (1987).
Vancanneyt et al., "Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation", Mol. Gen. Genet., 220, 245-250 (1990).
West et al., "Embryogenesis in Higher
Cai Tishu
Gu Weining
Pierce Dorothy A.
Zhao Zuo-Yu
Benzion Gary
Pioneer Hi-Bred International , Inc.
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